Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3736869..3737489 Replicon chromosome
Accession NZ_CP056466
Organism Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV214_RS18295 Protein ID WP_002892050.1
Coordinates 3737271..3737489 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV214_RS18290 Protein ID WP_003021733.1
Coordinates 3736869..3737243 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV214_RS18280 3732015..3733208 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV214_RS18285 3733231..3736380 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV214_RS18290 3736869..3737243 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV214_RS18295 3737271..3737489 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV214_RS18300 3737796..3738347 + 552 WP_003835924.1 maltose O-acetyltransferase -
HV214_RS18305 3738464..3738934 + 471 WP_003021724.1 YlaC family protein -
HV214_RS18310 3739013..3739153 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV214_RS18315 3739155..3739415 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
HV214_RS18320 3739604..3741157 + 1554 WP_046669815.1 EAL domain-containing protein -
HV214_RS18325 3741209..3741562 - 354 WP_003831033.1 DUF1428 family protein -
HV214_RS18330 3741627..3742256 - 630 WP_016149709.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163552 WP_002892050.1 NZ_CP056466:3737271-3737489 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163552 NZ_CP072680:c1354301-1354023 [Vibrio sp. SCSIO 43186]
ATGATTTTGTGGGAAGAAGAGTCTCTCAACGATCGCGAAAAGATCTTTGAGTTTCTCTACGACTTCAACCCCGAAGCTGC
TGACAAAACCGACGATATTATTGAAGCAAAAGTTGAGAACCTTTTAGAGCAACCTCATATGGGAGTCCAGCGTGACGGAA
TTAGAGGGCGATTGCTCATTATTCCTGAGATTTCGATGATTGTTTCCTACTGGGTTGAAGGTGATATCATTCGAGTTATG
CGCGTGCTACACCAAAAACAAAAATTCCCAACGGACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163552 WP_003021733.1 NZ_CP056466:3736869-3737243 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163552 NZ_CP072680:c1354582-1354298 [Vibrio sp. SCSIO 43186]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACGAAGCGACTAGCCCAACAAATGGCTGAAAGCCAAGGCAGAAC
ACTAAGTGATGCTTGTCGTGAACTGACTGAGCAACTGGCTGACCAACAGAGAAAGACTTTATCTCACGACGCATGGTTAA
CTGAACAAATTAACTTAGCTTTTGAGAAGTTTGACTCAGGAAAAGCCACTTTTATTGACCATGAGTCAGCTAAATCTCGT
ATGGACGAGCGTAAAGCTAAAATCCGTAACCGAGGCAAATTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References