Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3117843..3118433 Replicon chromosome
Accession NZ_CP056466
Organism Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag HV214_RS15355 Protein ID WP_003836692.1
Coordinates 3117843..3118175 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag HV214_RS15360 Protein ID WP_003836694.1
Coordinates 3118176..3118433 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV214_RS15330 3113596..3115083 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HV214_RS15340 3115399..3116349 - 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
HV214_RS15345 3116451..3117368 - 918 WP_181548239.1 nitrogen assimilation transcriptional regulator NAC -
HV214_RS15355 3117843..3118175 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HV214_RS15360 3118176..3118433 - 258 WP_003836694.1 hypothetical protein Antitoxin
HV214_RS15365 3119213..3120229 + 1017 WP_001407551.1 IS5-like element IS5 family transposase -
HV214_RS15370 3120947..3121468 + 522 WP_003036312.1 sigma-70 family RNA polymerase sigma factor -
HV214_RS15375 3121465..3122418 + 954 WP_046670485.1 fec operon regulator FecR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3119249..3120229 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T163551 WP_003836692.1 NZ_CP056466:c3118175-3117843 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T163551 NZ_CP072680:c1344559-1344263 [Vibrio sp. SCSIO 43186]
ATGCAAAAGAATAAATATAAGCTAAGTAAATTAGCACAGACCCATTTACAAAAAATCAAAAACTATACCGTCAACAATTT
CTCTGAGATGCAATGGCGAAGCTACAAAGATACCTTACTAACAGGGTTTCAAATGCTTGCTGACAACCCGGCTGTTGGTC
GCAGTTGCGATGAGATATACCCAAGTGGTTTTTATTTCCCAGTGGGTAAGCATACCGCTTACTTTACAAAAGAAGATGGC
TTCATTTTAGTTGTTGCTGTACTCGGTCAATCACAACTTCCGCAGAACCACCTGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT163551 WP_003836694.1 NZ_CP056466:c3118433-3118176 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT163551 NZ_CP072680:c1344808-1344566 [Vibrio sp. SCSIO 43186]
ATGCACACATTAACAGCAAATGATGCCAAACGTAATTTTGGTGAGTTACTTCTGAGCGCTCAACGTGAGCCAGTGAAAAT
TAGCAAAAATAGCAAAGATGCCGTAGTAGTTATGTCGATTAAGGACTATGAAGAGCTGGAAGCAATGAAAACTGATTACC
TGAGACATTGTTTCGAGTCAGCTAAGCAAGACTTGGTACAAGGCAATGTGGTTGATGGTGAAGACTTTTTAAACGCCTTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References