Detailed information of TA system TA163541

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 927453..928114
• Replicon: chromosome
• Accession: NZ_CP056466
• Organism: Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV214_RS04615
• Protein ID: WP_048997879.1
• Coordinates: 927791..928114 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A241Q747
• Locus tag: HV214_RS04610
• Protein ID: WP_048997880.1
• Coordinates: 927453..927770 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV214_RS04565	922570..923394	+	825	WP_101743252.1	DUF945 domain-containing protein	-
HV214_RS04570	923603..924316	+	714	WP_175329887.1	DeoR family transcriptional regulator	-
HV214_RS04575	924342..924878	+	537	WP_049002976.1	DUF4339 domain-containing protein	-
HV214_RS04580	924920..925357	+	438	WP_049002977.1	hypothetical protein	-
HV214_RS04585	925424..925834	+	411	WP_049002978.1	hypothetical protein	-
HV214_RS04590	925912..926151	+	240	WP_048998188.1	DUF905 domain-containing protein	-
HV214_RS04595	926254..926712	+	459	WP_049002979.1	antirestriction protein	-
HV214_RS04600	926728..927204	+	477	WP_049016517.1	RadC family protein	-
HV214_RS04605	927213..927434	+	222	WP_048997881.1	DUF987 domain-containing protein	-
HV214_RS04610	927453..927770	+	318	WP_048997880.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV214_RS04615	927791..928114	+	324	WP_048997879.1	TA system toxin CbtA family protein	Toxin
HV214_RS04620	928355..928891	-	537	WP_032949993.1	GNAT family N-acetyltransferase	-
HV214_RS04625	929500..930516	+	1017	WP_001407551.1	IS5-like element IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	899837..930516	30679
	-	flank	IS/Tn	-	-	929536..930516	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12455.32 Da
• Isoelectric Point: 6.4624

>T163541 WP_048997879.1 NZ_CP056466:927791-928114 [Citrobacter freundii]
MKSQPATTQRAAKPCLSPVDVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGITLADAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T163541 NZ_CP072653:c4650885-4650601 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11993.64 Da
• Isoelectric Point: 7.6976

>AT163541 WP_048997880.1 NZ_CP056466:927453-927770 [Citrobacter freundii]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGQFSDAECRKLDVAFPYFIRQMESMLTTGELNPRHTHCVTLYR
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT163541 NZ_CP072653:c4651117-4650875 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAACAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A241Q747