Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 874935..875589 Replicon chromosome
Accession NZ_CP056466
Organism Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag HV214_RS04320 Protein ID WP_003026936.1
Coordinates 875182..875589 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag HV214_RS04315 Protein ID WP_003026938.1
Coordinates 874935..875201 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV214_RS04290 870135..871568 - 1434 WP_046670318.1 6-phospho-beta-glucosidase BglA -
HV214_RS04295 871690..872418 - 729 WP_181213132.1 MurR/RpiR family transcriptional regulator -
HV214_RS04300 872471..872782 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
HV214_RS04305 872945..873604 + 660 WP_046670319.1 hemolysin III family protein -
HV214_RS04310 873698..874678 - 981 WP_003026944.1 tRNA-modifying protein YgfZ -
HV214_RS04315 874935..875201 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
HV214_RS04320 875182..875589 + 408 WP_003026936.1 protein YgfX Toxin
HV214_RS04325 875634..876155 - 522 WP_003026933.1 flavodoxin FldB -
HV214_RS04330 876269..877165 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
HV214_RS04335 877189..877902 + 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HV214_RS04340 877908..879641 + 1734 WP_016150943.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T163540 WP_003026936.1 NZ_CP056466:875182-875589 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T163540 NZ_CP072653:c4535156-4534623 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTATTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT163540 WP_003026938.1 NZ_CP056466:874935-875201 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT163540 NZ_CP072653:c4535433-4535167 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7


Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References