Detailed information of TA system TA163539

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 767354..768147
• Replicon: chromosome
• Accession: NZ_CP056466
• Organism: Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: HV214_RS03770
• Protein ID: WP_000854726.1
• Coordinates: 767354..767731 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: HV214_RS03775
• Protein ID: WP_175330022.1
• Coordinates: 767821..768147 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV214_RS03750	763631..765490	+	1860	WP_003838200.1	bifunctional glutathionylspermidine amidase/synthase	-
HV214_RS03755	765733..766575	-	843	WP_001548928.1	DUF4942 domain-containing protein	-
HV214_RS03760	766660..766857	-	198	WP_000839269.1	DUF957 domain-containing protein	-
HV214_RS03765	766869..767357	-	489	WP_001547769.1	hypothetical protein	-
HV214_RS03770	767354..767731	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
HV214_RS03775	767821..768147	-	327	WP_175330022.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HV214_RS03780	768166..768387	-	222	WP_000691994.1	DUF987 domain-containing protein	-
HV214_RS03785	768396..768872	-	477	WP_175330023.1	RadC family protein	-
HV214_RS03790	768888..769346	-	459	WP_000211838.1	antirestriction protein	-
HV214_RS03795	769444..769683	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HV214_RS03800	769760..770227	-	468	WP_001547765.1	hypothetical protein	-
HV214_RS03805	770251..770694	-	444	WP_175330024.1	hypothetical protein	-
HV214_RS03810	770694..770930	-	237	WP_001144031.1	hypothetical protein	-
HV214_RS03815	770971..771672	-	702	WP_175330025.1	WYL domain-containing protein	-
HV214_RS03820	771890..772711	-	822	WP_008324532.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	763631..809656	46025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T163539 WP_000854726.1 NZ_CP056466:c767731-767354 [Citrobacter freundii]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T163539 NZ_CP072653:3924224-3924442 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12278.00 Da
• Isoelectric Point: 7.7489

>AT163539 WP_175330022.1 NZ_CP056466:c768147-767821 [Citrobacter freundii]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDKTFPHFIRQMESMLTTGELSPNHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT163539 NZ_CP072653:3923823-3924197 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1EZ92