Detailed information of TA system TA163522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2288897..2289636
• Replicon: chromosome
• Accession: NZ_CP056455
• Organism: Enterobacter hormaechei strain RHBSTW-00492

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV218_RS11015
• Protein ID: WP_063139083.1
• Coordinates: 2288897..2289382 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: HV218_RS11020
• Protein ID: WP_003857131.1
• Coordinates: 2289370..2289636 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV218_RS10990	2284715..2285473	-	759	WP_032624253.1	trans-aconitate 2-methyltransferase	-
HV218_RS10995	2285558..2286142	-	585	WP_022648128.1	NUDIX hydrolase	-
HV218_RS11000	2286229..2286987	+	759	WP_022648129.1	DeoR/GlpR family DNA-binding transcription regulator	-
HV218_RS11005	2287092..2288384	+	1293	WP_045622665.1	glycoside hydrolase	-
HV218_RS11010	2288671..2288871	+	201	Protein_2144	hypothetical protein	-
HV218_RS11015	2288897..2289382	-	486	WP_063139083.1	GNAT family N-acetyltransferase	Toxin
HV218_RS11020	2289370..2289636	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
HV218_RS11025	2289700..2290629	-	930	WP_063139082.1	LysR family transcriptional regulator	-
HV218_RS11030	2290759..2292147	+	1389	WP_045622667.1	MFS transporter	-
HV218_RS11035	2292126..2292683	-	558	WP_045622668.1	OmpH family outer membrane protein	-
HV218_RS11040	2292804..2293940	-	1137	WP_045622670.1	type 1 fimbrial protein	-
HV218_RS11045	2293964..2294488	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2274988..2289636	14648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17544.22 Da
• Isoelectric Point: 9.6275

>T163522 WP_063139083.1 NZ_CP056455:c2289382-2288897 [Enterobacter hormaechei]
VGRVTPPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T163522 NZ_CP072635:662081-662305 [Chloracidobacterium sp. D]
GTGAGCGACCTACCTAAAATCTCTGGACGTGAGTTAGTCAAAGCTCTCAAGAAGATAGGTTATGAGCAAGACCGACAGCG
AGGCAGCCATATCATACTCCGGCAAATAGACCCTCCACACCGGAGAGTGACAGTGCCAGACCACAAAGAAATAGCAAAAG
GGACATTGTGGACAATAATCCGCGAAATCGGACTGACAATTGATGAGCTTAAGGACTTGTTATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT163522 WP_003857131.1 NZ_CP056455:c2289636-2289370 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163522 NZ_CP072635:661872-662084 [Chloracidobacterium sp. D]
ATGAAATATAGAGTATTCATTGAGCAGGATGAAGACAATGTGTATGTTGCAACAGCACCTTCTCTGCCCGGCTGCGTTTC
CCAGGGAAAAACTCGCTCTGAGGCTTTGGAAAACATTAAGGAAGCTATCACAGGATACCTGGAGAGCCTGAAAGCTCATG
GTGAACCTGTCCCGCCTCCAATTTCTGAAGAAATCGTGGATGTTTCATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9