Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4706169..4706785 Replicon chromosome
Accession NZ_CP056451
Organism Citrobacter freundii strain RHBSTW-00502

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HV221_RS22665 Protein ID WP_003028682.1
Coordinates 4706169..4706543 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HV221_RS22670 Protein ID WP_043018956.1
Coordinates 4706543..4706785 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV221_RS22650 4703672..4704574 + 903 WP_003847898.1 formate dehydrogenase O subunit beta -
HV221_RS22655 4704571..4705206 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV221_RS22660 4705203..4706132 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV221_RS22665 4706169..4706543 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HV221_RS22670 4706543..4706785 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HV221_RS22675 4706991..4707899 + 909 WP_003847901.1 alpha/beta hydrolase -
HV221_RS22680 4708050..4708991 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV221_RS22685 4709036..4709473 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV221_RS22690 4709470..4710342 - 873 WP_003028669.1 virulence factor BrkB family protein -
HV221_RS22695 4710336..4710935 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T163519 WP_003028682.1 NZ_CP056451:c4706543-4706169 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T163519 NZ_CP072633:c231150-230926 [Chloracidobacterium thermophilum]
GTGAGCGACCTACCTAAAATCTCTGGACGTGAATTAGTCAAAGCTCTCAAGAAGATAGGTTATGAGCAAGACCGACAGCG
AGGCAGCCATATCATACTCCGGCAAATAGACCCTCCACACCGGAGAGTGACAGTGCCAGACCACAAAGAAATAGCAAAAG
GGACATTGCGGACAATAATCCGCGAAATCGGACTGACAATTGATGAGCTTAAGGACTTGTTATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT163519 WP_043018956.1 NZ_CP056451:c4706785-4706543 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT163519 NZ_CP072633:c231359-231147 [Chloracidobacterium thermophilum]
ATGAAATATCGAGTATTCATTGAGCAGGATGAAGACAATGTGTATATTGCGACAGTACCTTCTCTACCCGGCTGTGTTTC
CCAGGGAAAAACTCGCTCTGAGGCTTTGGAAAACATTAAGGAAGCTATCGCAGGATACCTAGAGAGCCTGAAAGCTCATG
GTGAACCTGTCCCGCCTCCAATTTCTGAAGAAATCGTGGATGTTTCATCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References