Detailed information of TA system TA163518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4554647..4555313
• Replicon: chromosome
• Accession: NZ_CP056451
• Organism: Citrobacter freundii strain RHBSTW-00502

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: HV221_RS21985
• Protein ID: WP_048216973.1
• Coordinates: 4554954..4555313 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0J1QJH0
• Locus tag: HV221_RS21980
• Protein ID: WP_032938223.1
• Coordinates: 4554647..4554964 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV221_RS21945	4549826..4550623	+	798	WP_003841402.1	PTS mannose/fructose/sorbose transporter subunit IIC	-
HV221_RS21950	4550635..4551459	+	825	WP_048216971.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HV221_RS21955	4551527..4552750	+	1224	WP_003031639.1	L-sorbose 1-phosphate reductase	-
HV221_RS21960	4552752..4553564	+	813	WP_048216972.1	shikimate 5-dehydrogenase	-
HV221_RS21965	4553614..4553970	-	357	WP_032938220.1	hypothetical protein	-
HV221_RS21970	4554102..4554275	+	174	WP_032938222.1	hypothetical protein	-
HV221_RS21975	4554346..4554507	+	162	WP_003841416.1	phage protein	-
HV221_RS21980	4554647..4554964	-	318	WP_032938223.1	helix-turn-helix domain-containing protein	Antitoxin
HV221_RS21985	4554954..4555313	-	360	WP_048216973.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV221_RS21990	4555527..4556216	+	690	WP_003841421.1	dipeptidase PepE	-
HV221_RS21995	4556282..4557913	-	1632	WP_003031618.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13410.46 Da
• Isoelectric Point: 10.2555

>T163518 WP_048216973.1 NZ_CP056451:c4555313-4554954 [Citrobacter freundii]
MTKPLYWVGQARKDLLAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGSAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T163518 NZ_CP072630:2467982-2468383 [Leptospira borgpetersenii]
ATGACTCAATACTTATTAGATACGAATATTTGCATTTATATAATAAACCAAAAGCCGAGATCTGTTTATAAAAAATTTAA
AAAGATCAAGTTAGAAAACATATTTATTTCATCAATTACGGAATTTGAATTAAAGTATGGTGTTCAAAAAAGTCTTCATC
TCGAAAGGAACTTAAAAATTTTAGAAGAGTTTTTAAGTTATTTGAATATTCTTTCATTTGTTAGTGAAGACGCAAATAAA
GCTGCAAAAATTCGAGTAGAATTAGACAAAGTAGGGAAACCAATCGGTCCTTTCGATCTTTTAATAGCTTCTCAAGCGTT
ATCGAATAAACTTACTCTCGTAACAAACAATGAAAAAGAGTTTACTCGGATCAAAGATATTAAAATTGCAAACTGGCTTT
AA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11451.29 Da
• Isoelectric Point: 6.9796

>AT163518 WP_032938223.1 NZ_CP056451:c4554964-4554647 [Citrobacter freundii]
MSHEVEASSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGEEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT163518 NZ_CP072630:2467755-2467985 [Leptospira borgpetersenii]
ATGAATAAAGCTAAAATTTTTAAAAATGGGGATAGTCAAGCAATTCGTTTACCGAAAGACTATCGTTTCAAAGGCAAAGA
AGTCTATATACGAAGGGATGGAGATAATGTTATCTTAACGCCAATAAACGATGCGGTTGATCGTTTTTGGAATACGGTTA
ATAGCTTTTCTGACGATCTTATACTTGATCGAAACCAACCAAACGAGTATGGCAAACGAAATAAAATATGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1QJH0