Detailed information of TA system TA163517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4330461..4330977
• Replicon: chromosome
• Accession: NZ_CP056451
• Organism: Citrobacter freundii strain RHBSTW-00502

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HV221_RS20880
• Protein ID: WP_048216920.1
• Coordinates: 4330461..4330745 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0J1MV10
• Locus tag: HV221_RS20885
• Protein ID: WP_003839576.1
• Coordinates: 4330735..4330977 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV221_RS20865	4326786..4327370	+	585	WP_003839584.1	fructose PTS transporter subunit IIA	-
HV221_RS20870	4327748..4329886	+	2139	WP_003839582.1	anaerobic ribonucleoside-triphosphate reductase	-
HV221_RS20875	4329993..4330457	+	465	WP_032937736.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HV221_RS20880	4330461..4330745	-	285	WP_048216920.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV221_RS20885	4330735..4330977	-	243	WP_003839576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HV221_RS20890	4331055..4332968	-	1914	WP_048216921.1	BglG family transcription antiterminator	-
HV221_RS20895	4332990..4333730	-	741	WP_048216922.1	KDGP aldolase family protein	-
HV221_RS20900	4333727..4334845	-	1119	WP_048216923.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
HV221_RS20905	4334829..4335962	-	1134	WP_048216924.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10905.80 Da
• Isoelectric Point: 10.1715

>T163517 WP_048216920.1 NZ_CP056451:c4330745-4330461 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDKVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T163517 NZ_CP072630:480180-480503 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTAGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.34 Da
• Isoelectric Point: 5.0651

>AT163517 WP_003839576.1 NZ_CP056451:c4330977-4330735 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT163517 NZ_CP072630:479938-480186 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MV10