Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3858039..3858718 Replicon chromosome
Accession NZ_CP056451
Organism Citrobacter freundii strain RHBSTW-00502

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HV221_RS18745 Protein ID WP_003031349.1
Coordinates 3858377..3858718 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HV221_RS18740 Protein ID WP_003031347.1
Coordinates 3858039..3858356 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV221_RS18715 3855588..3856619 + 1032 WP_001395480.1 IS630-like element ISEc33 family transposase -
HV221_RS18720 3856600..3856824 + 225 WP_181595255.1 DUF905 family protein -
HV221_RS18725 3856838..3857299 + 462 WP_003031344.1 antirestriction protein -
HV221_RS18730 3857315..3857791 + 477 WP_003031345.1 RadC family protein -
HV221_RS18735 3857800..3858021 + 222 WP_003031346.1 DUF987 domain-containing protein -
HV221_RS18740 3858039..3858356 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV221_RS18745 3858377..3858718 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV221_RS18755 3859348..3859812 - 465 WP_048216807.1 isoprenylcysteine carboxylmethyltransferase family protein -
HV221_RS18760 3860005..3861453 - 1449 WP_048217399.1 EAL domain-containing protein -
HV221_RS18765 3861795..3862268 - 474 WP_003838775.1 hypothetical protein -
HV221_RS18770 3862243..3862974 - 732 WP_048216808.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3842525..3870615 28090
- flank IS/Tn - - 3855588..3856619 1031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T163515 WP_003031349.1 NZ_CP056451:3858377-3858718 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T163515 NZ_CP072628:2468396-2468797 [Leptospira borgpetersenii]
ATGACTCAATACTTATTAGATACGAATATTTGCATTTATATAATAAACCAAAAGCCGAGATCTGTTTATAAAAAATTTAA
AAAGATCAAGTTAGAAAACATATTTATTTCATCAATTACGGAATTTGAATTAAAGTATGGTGTTCAAAAAAGTCTTCATC
TCGAAAGGAACTTAAAAATTTTAGAAGAGTTTTTAAGTTATTTGAATATTCTTTCATTTGTTAGTGAAGACGCAAATAAA
GCTGCAAAAATTCGAGTAGAATTAGACAAAGTAGGGAAACCAATCGGTCCTTTCGATCTTTTAATAGCTTCTCAAGCGTT
ATCGAATAAACTTACTCTCGTAACAAACAATGAAAAAGAGTTTACTCGGATCAAAGATATTAAAATTGCAAACTGGCTTT
AA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT163515 WP_003031347.1 NZ_CP056451:3858039-3858356 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT163515 NZ_CP072628:2468169-2468399 [Leptospira borgpetersenii]
ATGAATAAAGCTAAAATTTTTAAAAATGGGGATAGTCAAGCAATTCGTTTACCGAAAGACTATCGTTTCAAAGGCAAAGA
AGTCTATATACGAAGGGATGGAGATAATGTTATCTTAACGCCAATAAACGATGCGGTTGATCGTTTTTGGAATACGGTTA
ATAGCTTTTCTGACGATCTTATACTTGATCGAAACCAACCAAACGAGTATGGCAAACGAAATAAAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References