Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3698112..3698732 Replicon chromosome
Accession NZ_CP056451
Organism Citrobacter freundii strain RHBSTW-00502

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV221_RS17945 Protein ID WP_002892050.1
Coordinates 3698514..3698732 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV221_RS17940 Protein ID WP_003021733.1
Coordinates 3698112..3698486 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV221_RS17930 3693258..3694451 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV221_RS17935 3694474..3697623 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV221_RS17940 3698112..3698486 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV221_RS17945 3698514..3698732 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV221_RS17950 3699039..3699590 + 552 WP_032936967.1 maltose O-acetyltransferase -
HV221_RS17955 3699707..3700177 + 471 WP_003021724.1 YlaC family protein -
HV221_RS17960 3700256..3700396 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV221_RS17965 3700398..3700658 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
HV221_RS17970 3700847..3702401 + 1555 Protein_3519 EAL domain-containing protein -
HV221_RS17975 3702453..3702806 - 354 WP_003831033.1 DUF1428 family protein -
HV221_RS17980 3702871..3703491 - 621 WP_048216756.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163514 WP_002892050.1 NZ_CP056451:3698514-3698732 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163514 NZ_CP072628:480179-480502 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTAGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163514 WP_003021733.1 NZ_CP056451:3698112-3698486 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163514 NZ_CP072628:479937-480185 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References