Detailed information of TA system TA163499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 58898..59423
• Replicon: plasmid pRHBSTW-00510_3
• Accession: NZ_CP056434
• Organism: Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: HV225_RS27815
• Protein ID: WP_004197633.1
• Coordinates: 59118..59423 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1D8K3R4
• Locus tag: HV225_RS27810
• Protein ID: WP_006788213.1
• Coordinates: 58898..59116 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV225_RS27765	54288..54491	-	204	WP_004197809.1	hypothetical protein	-
HV225_RS27770	54505..54708	-	204	WP_004197808.1	hemolysin expression modulator Hha	-
HV225_RS27775	54742..55110	-	369	WP_004197807.1	hypothetical protein	-
HV225_RS27780	55154..55648	-	495	WP_020805591.1	hypothetical protein	-
HV225_RS27785	55679..56251	-	573	WP_020805590.1	hypothetical protein	-
HV225_RS27790	56248..56496	-	249	WP_000272716.1	hypothetical protein	-
HV225_RS27795	56933..57622	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
HV225_RS27800	57654..58343	-	690	WP_004193995.1	hypothetical protein	-
HV225_RS27805	58483..58728	+	246	Protein_60	hypothetical protein	-
HV225_RS27810	58898..59116	+	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV225_RS27815	59118..59423	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV225_RS27820	59481..59792	+	312	WP_023287114.1	hypothetical protein	-
HV225_RS27825	59842..60177	+	336	WP_009309920.1	hypothetical protein	-
HV225_RS27830	60211..61227	+	1017	WP_181467684.1	hypothetical protein	-
HV225_RS27835	61271..61432	+	162	WP_009483881.1	hypothetical protein	-
HV225_RS27840	61425..62204	+	780	WP_100699031.1	site-specific integrase	-
HV225_RS27845	62363..63325	-	963	WP_096835037.1	Abi family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..137885	137885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T163499 WP_004197633.1 NZ_CP056434:59118-59423 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T163499 NZ_CP072618:c2829796-2829473 [Leptospira borgpetersenii]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTAGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7405

>AT163499 WP_006788213.1 NZ_CP056434:58898-59116 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT163499 NZ_CP072618:c2830038-2829790 [Leptospira borgpetersenii]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5

Antitoxin

• AlphaFold DB: A0A1D8K3R4