Detailed information of TA system TA163497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 104206..104728
• Replicon: plasmid pRHBSTW-00510_2
• Accession: NZ_CP056433
• Organism: Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: HV225_RS26990
• Protein ID: WP_004187019.1
• Coordinates: 104444..104728 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HV225_RS26985
• Protein ID: WP_004187025.1
• Coordinates: 104206..104454 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV225_RS26965	99240..100001	-	762	WP_023329011.1	histidine utilization repressor	-
HV225_RS26970	100104..101471	+	1368	WP_023329010.1	formimidoylglutamate deiminase	-
HV225_RS26975	101606..102631	+	1026	WP_001101446.1	IS110 family transposase	-
HV225_RS26980	102950..103918	-	969	WP_072206835.1	IS5 family transposase	-
HV225_RS26985	104206..104454	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HV225_RS26990	104444..104728	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV225_RS26995	104744..106087	-	1344	WP_032738442.1	ISNCY family transposase	-
HV225_RS27000	106889..107148	+	260	Protein_116	hypothetical protein	-
HV225_RS27005	107259..107729	-	471	WP_023329005.1	RES family NAD+ phosphorylase	-
HV225_RS27010	107726..108169	-	444	WP_023329004.1	DUF2384 domain-containing protein	-
HV225_RS27015	108291..108978	-	688	Protein_119	DUF4113 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..206782	206782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T163497 WP_004187019.1 NZ_CP056433:104444-104728 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T163497 NZ_CP072616:97715-97990 [Sulfitobacter sp. CB2047]
GTGCCTGATCTCGAATGGAAGGCCACGGCAATCGCAGATCTACTTGCGATTATCGACTACATCTCTGACGACAATCCAGA
TGCGGCCCAAGCTCTCAAGGACGAAATCGAGGTTAAGACATCACGATTGCCAGAGCACCCCCAGATGTACCGCGTGGGAC
GTGTTGACGGCACCCGTGAAATGGTTGTGCGTCCAAATTACATCGTGATTTATGCTGACGACCCAAGTTCCATAGTGATC
TTGCGAGTGCTGCACGCAGCTCAGCAGTGGCCATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT163497 WP_004187025.1 NZ_CP056433:104206-104454 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT163497 NZ_CP072616:97429-97722 [Sulfitobacter sp. CB2047]
ATGCCCGCTCAAACATCTATGATCCATGTCCGTGTTGATGATCAGCTTAAAGCGCAGGCCTTGGATGCGCTCTCGGGCGT
CGGCCTGACGCTCTCGGATGCCGTACGCATCCTTTTGACGCGCGTGGCCGCAGAGGGCGGGTTGCCAGCGGGACTGACCG
CAGATCCAGACGCCTATGACGCATGGTTCCGTTCCAAAGTGCAGGAAGCATTGGACGACTCAAAGCCGACAACTCCGCAG
GCTCAGGCAATGCAAGACGCTCAGGCGCTGATTGATAAGAAGCGTCGTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4