Detailed information of TA system TA163496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 85857..86593
• Replicon: plasmid pRHBSTW-00510_2
• Accession: NZ_CP056433
• Organism: Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV225_RS26890
• Protein ID: WP_023329018.1
• Coordinates: 85857..86339 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HV225_RS26895
• Protein ID: WP_003026799.1
• Coordinates: 86327..86593 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV225_RS26870	81135..81953	+	819	WP_023307220.1	abortive infection family protein	-
HV225_RS26875	82024..83367	-	1344	WP_077263966.1	ISNCY family transposase	-
HV225_RS26880	83593..84225	+	633	WP_001567369.1	hypothetical protein	-
HV225_RS26885	84254..85657	-	1404	WP_181467669.1	ISNCY-like element ISKpn21 family transposase	-
HV225_RS26890	85857..86339	-	483	WP_023329018.1	GNAT family N-acetyltransferase	Toxin
HV225_RS26895	86327..86593	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HV225_RS26900	87697..87876	+	180	Protein_96	transposase	-
HV225_RS26905	87843..87977	+	135	WP_109241503.1	integrase core domain-containing protein	-
HV225_RS26910	88129..88938	-	810	WP_023329017.1	N-formylglutamate deformylase	-
HV225_RS26915	88931..90139	-	1209	WP_023329016.1	imidazolonepropionase	-
HV225_RS26920	90151..91545	-	1395	WP_032738443.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..206782	206782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17255.84 Da
• Isoelectric Point: 7.8840

>T163496 WP_023329018.1 NZ_CP056433:c86339-85857 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAEGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T163496 NZ_CP072612:3432195-3432941 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT163496 WP_003026799.1 NZ_CP056433:c86593-86327 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163496 NZ_CP072612:3432942-3433112 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66