Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5264418..5265043 Replicon chromosome
Accession NZ_CP056432
Organism Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV225_RS26055 Protein ID WP_002882817.1
Coordinates 5264418..5264801 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV225_RS26060 Protein ID WP_004150355.1
Coordinates 5264801..5265043 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV225_RS26040 5261784..5262686 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV225_RS26045 5262683..5263318 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV225_RS26050 5263315..5264244 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV225_RS26055 5264418..5264801 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV225_RS26060 5264801..5265043 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV225_RS26065 5265248..5266165 + 918 WP_023302328.1 alpha/beta hydrolase -
HV225_RS26070 5266179..5267120 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV225_RS26075 5267165..5267602 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV225_RS26080 5267599..5268459 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV225_RS26085 5268453..5269052 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T163495 WP_002882817.1 NZ_CP056432:c5264801-5264418 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163495 NZ_CP072612:c254410-254060 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163495 WP_004150355.1 NZ_CP056432:c5265043-5264801 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163495 NZ_CP072612:c254697-254416 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References