Detailed information of TA system TA163494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4911323..4913004
• Replicon: chromosome
• Accession: NZ_CP056432
• Organism: Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: HV225_RS24370
• Protein ID: WP_004146663.1
• Coordinates: 4911323..4912240 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: HV225_RS24375
• Protein ID: WP_004177809.1
• Coordinates: 4912246..4913004 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV225_RS24350	4906629..4908149	+	1521	WP_032738364.1	sugar ABC transporter ATP-binding protein	-
HV225_RS24355	4908146..4909144	+	999	WP_012737175.1	ribose ABC transporter permease	-
HV225_RS24360	4909175..4910116	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
HV225_RS24365	4910191..4911198	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
HV225_RS24370	4911323..4912240	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HV225_RS24375	4912246..4913004	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HV225_RS24380	4913090..4913818	-	729	WP_004177810.1	response regulator transcription factor	-
HV225_RS24385	4914091..4916238	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
HV225_RS24390	4916468..4917154	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T163494 WP_004146663.1 NZ_CP056432:c4912240-4911323 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T163494 NZ_CP072610:2314918-2315214 [Pseudomonas wenzhouensis]
ATGGAAAAGCGCAAACCCCATTTCAGGCTTGAGTTGGTGAAACAGGCTGTAGCCGACCAGCGGTATCGCTTCACCCGCGT
AGCCTTGGAAGGAGGCGCTGCGCTGGGCATGGAGATGGCCGACATGCTGGCCGTCATCAGTGCCCTGAGCAGCCGCGACT
TTTTCAAGAGCATGACAACCTATGCGGATCATACTACCTGGCAGGACGTTTACCGACCCGATACCGAATTCGGGCAGGTC
TATCTGAAGTTCACGCTGGTGGCCGATCTGCTAATCGTTTCCTTTAAGGAGAAGTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT163494 WP_004177809.1 NZ_CP056432:c4913004-4912246 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT163494 NZ_CP072610:2315217-2315618 [Pseudomonas wenzhouensis]
ATGAAATGTCCAGTATGCGGCGGGGCGGAACTGGTTCACGACACCCGCGATATGCCCTTCACCTACAAGGGGCAAACCAC
CCAAATTACTGCAGTAACGGCTGACTGGTGCGATGCCTGTGGCGAGTCACTGACTGGGCCGGCTGAAAGTGAGCGCGTTA
TGCGTGCCATGAACGAGTTTCGCCAGCAGGTGAACGCCCAGGATGGCAATCAGGAGCTGATTCGCAGCGTGCGTAAGCAG
TTGCGGTTGAGCCAGCGCGAAGCGGCCGAGCTGTTCGGTGGTGGGCCGAATGCGTTTTCTCGCTACGAGCGAGGCAGCAC
AGAAGCACCGCAACCGCTGGTGCAGTTGTTCAAGATCCTGGGCCGACACCCGGAGTTGATTAACGAGCTGCGTGCAGGTT
GA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05