Detailed information of TA system TA163492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4651782..4652592
• Replicon: chromosome
• Accession: NZ_CP056432
• Organism: Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: HV225_RS23115
• Protein ID: WP_014908042.1
• Coordinates: 4651782..4652315 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: HV225_RS23120
• Protein ID: WP_023343051.1
• Coordinates: 4652326..4652592 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV225_RS23110	4650613..4651734	+	1122	WP_012737335.1	cupin domain-containing protein	-
HV225_RS23115	4651782..4652315	-	534	WP_014908042.1	GNAT family N-acetyltransferase	Toxin
HV225_RS23120	4652326..4652592	-	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
HV225_RS23125	4652695..4654128	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
HV225_RS23130	4654118..4654801	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HV225_RS23135	4654974..4656359	+	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
HV225_RS23140	4656377..4656721	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T163492 WP_014908042.1 NZ_CP056432:c4652315-4651782 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T163492 NZ_CP072610:2170261-2170554 [Pseudomonas wenzhouensis]
GTGCCACGACTGATCGTCACGGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCGCGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCATTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATTGAGTTTGGTGACTCAGGTTACGTCGCGTTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT163492 WP_023343051.1 NZ_CP056432:c4652592-4652326 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT163492 NZ_CP072610:2170001-2170273 [Pseudomonas wenzhouensis]
ATGGCCACTTCCATCAAAATTGATGACAAGCTAAAAGGCCGCATTCAGCAGTTGGCGAGCCAACGCCAGCGCTCGTCTCA
CTGGATCATGCGCGAGGCAATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAGCAGGAAGCCCTGGCAT
CGTGGGCGGCATATCAGGAAACCGGACGGCATTTGACCAGCCAAGAAACTCGGACCTGGCTCGGTAGCTGGGGAACAGAT
ACTGAGGCAGAGTTGCCCAAGTGCCACGACTGA

Structures

Toxin

• AlphaFold DB: A0A332NV98