Detailed information of TA system TA163491

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4219874..4220580
• Replicon: chromosome
• Accession: NZ_CP056432
• Organism: Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV225_RS21085
• Protein ID: WP_024622903.1
• Coordinates: 4220212..4220580 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A485WAM6
• Locus tag: HV225_RS21080
• Protein ID: WP_023302278.1
• Coordinates: 4219874..4220191 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV225_RS21035	4215015..4215839	+	825	WP_023302271.1	DUF945 domain-containing protein	-
HV225_RS21040	4216048..4216758	+	711	WP_023302272.1	DeoR family transcriptional regulator	-
HV225_RS21045	4216784..4217320	+	537	WP_023302273.1	DUF4339 domain-containing protein	-
HV225_RS21050	4217362..4217799	+	438	WP_023301392.1	hypothetical protein	-
HV225_RS21055	4217866..4218276	+	411	WP_023302274.1	hypothetical protein	-
HV225_RS21060	4218354..4218590	+	237	WP_181467588.1	DUF905 domain-containing protein	-
HV225_RS21065	4218677..4219135	+	459	WP_023302275.1	antirestriction protein	-
HV225_RS21070	4219144..4219626	+	483	WP_023302276.1	RadC family protein	-
HV225_RS21075	4219635..4219856	+	222	WP_023302277.1	DUF987 domain-containing protein	-
HV225_RS21080	4219874..4220191	+	318	WP_023302278.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV225_RS21085	4220212..4220580	+	369	WP_024622903.1	TA system toxin CbtA family protein	Toxin
HV225_RS21090	4220577..4220918	+	342	WP_024622904.1	hypothetical protein	-
HV225_RS21095	4221041..4223686	-	2646	WP_024622905.1	LuxR family transcriptional regulator	-
HV225_RS21100	4224060..4225019	+	960	WP_023302280.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.82 Da
• Isoelectric Point: 7.2897

>T163491 WP_024622903.1 NZ_CP056432:4220212-4220580 [Klebsiella pneumoniae]
MKNLPATISQAAKPCLSPVAVWQMLLTHLLEQHYGLMLSDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRYSYREVSNIVLSRSRI

Nucleotide

• Length: 369 bp

>T163491 NZ_CP072610:2167747-2168151 [Pseudomonas wenzhouensis]
ATGCTGAAGTACATGCTTGATACCAACATCTGCATTTTCACGATCAAGAATCGTCCTGAGCAGGTGCGGGAAGCCTTCAA
GCGCCATTCAGGGCAGCTTAGCATCAGCACTGTCACCCTGATGGAGCTTATTTACGGCGCCGAGAAGTCCGCTAATCCCG
AGCGTAATCTGGCGGACGTTGAGGGTTTCGCTGCGCGCCTGGAAGTACTGCCTTACGACGCTCAAGCAGCGGCTCATTCC
GGGCAATTGCGAGCTGAGCTGGCTCGTATCGGCAAGCCTATTGGCCCGTATGATCAGATGATTGCAGGACACGCACGTGC
CCAGGGACTGATTCTGGTCACGAATAATCTTCGTGAGTTCGAGCGAGTTCCAGGTTTGCGCGTCGAGGATTGGGTGAACG
CCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11860.67 Da
• Isoelectric Point: 6.4629

>AT163491 WP_023302278.1 NZ_CP056432:4219874-4220191 [Klebsiella pneumoniae]
MSNPTWGLLRDITPCLGARLVQEGNRLYYLVDRASITGKFSDAEILKLDVVFPHFISQMESMLTIGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT163491 NZ_CP072610:2167517-2167747 [Pseudomonas wenzhouensis]
ATGGAACAAGGTGCAGTTTTTCAGAGCAACCGCAGTCAGGCGGTGCGTTTGCCCAAGGCGGTTGCGCTGCCTGAGGATGT
GAAGCGGGTGGATGTAGTGGTAGTTGGTCGAGCCCGAATCATCACGCCGGCAGGCGAGGCCTGGGACCTCTGGTTCGATG
GCGAAGGTGTGACCGCTGACTACATGGCGGATCGCGAGCAGCCTGCAGATCAAGAGCGAGATGCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A485WAM6