Detailed information of TA system TA163489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2570717..2572305
• Replicon: chromosome
• Accession: NZ_CP056432
• Organism: Klebsiella pneumoniae strain RHBSTW-00510

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A485W5A2
• Locus tag: HV225_RS12710
• Protein ID: WP_023342812.1
• Coordinates: 2570717..2572039 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HV225_RS12715
• Protein ID: WP_024622638.1
• Coordinates: 2572039..2572305 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV225_RS12695	2565773..2568274	+	2502	WP_023342811.1	fimbrial biogenesis outer membrane usher protein	-
HV225_RS12700	2568262..2569266	+	1005	WP_004224758.1	type 1 fimbrial protein	-
HV225_RS12705	2569408..2570700	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HV225_RS12710	2570717..2572039	-	1323	WP_023342812.1	type II toxin-antitoxin system HipA family toxin	Toxin
HV225_RS12715	2572039..2572305	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HV225_RS12720	2572570..2572896	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
HV225_RS12725	2572893..2573393	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HV225_RS12730	2573371..2574750	-	1380	WP_004184021.1	purine permease	-
HV225_RS12735	2575096..2576250	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HV225_RS12740	2576231..2577154	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2373106..2605962	232856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5358

>T163489 WP_023342812.1 NZ_CP056432:c2572039-2570717 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEVNAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T163489 NZ_CP072610:c116146-115859 [Pseudomonas wenzhouensis]
ATGACCTATAGCCTCGAATTTGATGCGCGGGCGCTGAAGGAGTGGCACAAGTTGGGGGATACCGTCCGGGAGCAGCTCAA
GAAAAAGCTCGCGGAGGTGTTGGTCAATCCGCGTGTTGAGGCAAATCGCCTTCATTCACTTCCCGACTGCTACAAGATCA
AACTGCGCAGCAGCGGTTATCGCCTGGTGTATCAGGTGATCGACCATGAGGTGGTGGTGTTTGTCGTGGCCGTGGATCGG
CGTGAACGCGAGCAGGCCTATCGCAAGGCCGCTGAGCGGCTCAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT163489 WP_024622638.1 NZ_CP056432:c2572305-2572039 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT163489 NZ_CP072610:c116375-116136 [Pseudomonas wenzhouensis]
ATGGCTTCGATCAATATCCGCATCGACGACGAGCTGAAAGTCCGCGCGTACCGGGAACTGGAAAAGCTTGGCGTCACTCC
TTCTGAGCTGATGCGCCAAGCTCTGCAATACGTGGCCGAACGCGGTCAGCTGCCCTTCCGTCCGGTACTGATGACCGAGG
AAGATGAGGCCCTGATCGCCACTGTGCGTGAGCGCCTTGCTGCTCCCCAGCGCGTGAAGGTTTCGCTGGATGACCTATAG

Structures

Toxin

• AlphaFold DB: A0A485W5A2