Detailed information of TA system TA163475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 25258..25901
• Replicon: plasmid pRHBSTW-00515_2
• Accession: NZ_CP056426
• Organism: Klebsiella pneumoniae strain RHBSTW-00515

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A3Q8D6L7
• Locus tag: HV227_RS27515
• Protein ID: WP_020804312.1
• Coordinates: 25485..25901 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HV227_RS27510
• Protein ID: WP_001261276.1
• Coordinates: 25258..25488 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV227_RS27470	20798..20911	+	114	WP_014343462.1	hypothetical protein	-
HV227_RS27475	21040..21297	+	258	WP_009310077.1	hypothetical protein	-
HV227_RS27480	21355..22134	-	780	WP_023287113.1	site-specific integrase	-
HV227_RS27485	22332..23348	-	1017	WP_009309921.1	hypothetical protein	-
HV227_RS27490	23382..23717	-	336	WP_009309920.1	hypothetical protein	-
HV227_RS27495	23767..24078	-	312	WP_023287114.1	hypothetical protein	-
HV227_RS27500	24136..24441	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
HV227_RS27505	24443..24661	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
HV227_RS27510	25258..25488	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV227_RS27515	25485..25901	+	417	WP_020804312.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV227_RS27520	25942..26820	-	879	WP_009309916.1	restriction endonuclease	-
HV227_RS27525	27482..28543	+	1062	Protein_32	HlyD family secretion protein	-
HV227_RS27530	28692..29672	-	981	WP_009310076.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..220501	220501
	-	flank	IS/Tn	-	-	19422..20402	980
	-	flank	IS/Tn	-	-	28692..29672	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15154.59 Da
• Isoelectric Point: 7.8637

>T163475 WP_020804312.1 NZ_CP056426:25485-25901 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T163475 NZ_CP072602:c86321-85983 [Burkholderia latens]
ATGGTTGACATACTGAAATCCATGCCCTACAGTTCGCCTTCGTACAGTATCCGGACCACAGACGCCTTCGACGCGTGGTT
TGCCGCCCTTACGGATCGCGTCGCGCGGCGCCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAACCCGGGCGACT
GGAAATCCGCCGGCTCACCGATCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTACTACGCGAGGCGCGAA
ACGATATGGGTGATCCTGCTCTGCGGCGGTGACAAGTCGACACAACAGGCGGACATCCGCGCCGCGCACGCGATGCTCGA
ACGGCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT163475 WP_001261276.1 NZ_CP056426:25258-25488 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT163475 NZ_CP072602:c85979-85680 [Burkholderia latens]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGACGACGACATGGCCGAGTATTTCGAGGC
CTGTCTGCAGGAAGCCGGCGACGACCCGGCCTTCATCGCGCATGCGCTTGGCGTGATCGCGCGCGCGCGCGGGATGTCGC
AGGTCGCGCGCGACGCCGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAGCTGCACGGCGCGAAGGCGCACGCGTGA

Structures

Toxin

• AlphaFold DB: A0A3Q8D6L7

Antitoxin

• AlphaFold DB: A0A387K023