Detailed information of TA system TA163473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5416418..5417043
• Replicon: chromosome
• Accession: NZ_CP056425
• Organism: Klebsiella pneumoniae strain RHBSTW-00515

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV227_RS27000
• Protein ID: WP_062921072.1
• Coordinates: 5416418..5416801 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: HV227_RS27005
• Protein ID: WP_004150355.1
• Coordinates: 5416801..5417043 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV227_RS26985	5413784..5414686	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
HV227_RS26990	5414683..5415318	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
HV227_RS26995	5415315..5416244	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
HV227_RS27000	5416418..5416801	-	384	WP_062921072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV227_RS27005	5416801..5417043	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV227_RS27010	5417248..5418165	+	918	WP_048266089.1	alpha/beta hydrolase	-
HV227_RS27015	5418179..5419120	-	942	WP_012543287.1	fatty acid biosynthesis protein FabY	-
HV227_RS27020	5419165..5419602	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV227_RS27025	5419599..5420459	-	861	WP_004146232.1	virulence factor BrkB family protein	-
HV227_RS27030	5420453..5421052	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T163473 WP_062921072.1 NZ_CP056425:c5416801-5416418 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDALILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T163473 NZ_CP072600:c5617654-5617379 [Leptothermofonsia sichuanensis PKUAC-SCTE412]
ATGGAATACGAATGGGATGAAGCAAAGCGTCTCGCCAATCTTCGTAAGCATGGAATTGACTTTATTGATGTCCCAGCCAT
ATTTGGTGGCGACACTGTAACTGTTGAGGATGATCGCTATAGCTATGGGGAGCAGCGGTTTATCACATTTGGCTTGTTGC
AAGGGCGAGTGATTGCTGTTGTCCATACCGAACAAGAGGATTATACCCGTATTATTTCCGCAAGAAAGGCAACGAAATAT
GAACAGAGAGCCTACTTCGAGCAACTCTCAAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT163473 WP_004150355.1 NZ_CP056425:c5417043-5416801 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT163473 NZ_CP072600:c5617359-5617138 [Leptothermofonsia sichuanensis PKUAC-SCTE412]
ATGACGGACGAAGATATTGATTTCTCAGACTGCCCAGAAGTTACACCAGAGATGTTTGCAAAAGCAATAGTGCGACGTGG
TTTACCCGTTGCAAAAGCAAAGACCCAGGTTACACTCCGTATCGATAGCGATGTTTTGGATTGGTTTAAGTCTCAGGGAC
GTGGCTATCAAACTCAAATCAATCAGTTGTTGCGGGCTTATATGGAAGCACATCAGCGGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9