Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4160386..4161005 Replicon chromosome
Accession NZ_CP056425
Organism Klebsiella pneumoniae strain RHBSTW-00515

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV227_RS20975 Protein ID WP_002892050.1
Coordinates 4160787..4161005 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HV227_RS20970 Protein ID WP_002892066.1
Coordinates 4160386..4160760 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV227_RS20960 4155538..4156731 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV227_RS20965 4156754..4159900 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HV227_RS20970 4160386..4160760 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HV227_RS20975 4160787..4161005 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV227_RS20980 4161164..4161730 + 567 WP_002892030.1 maltose O-acetyltransferase -
HV227_RS20985 4161702..4161842 - 141 WP_004147370.1 hypothetical protein -
HV227_RS20990 4161863..4162333 + 471 WP_002892026.1 YlaC family protein -
HV227_RS20995 4162308..4163759 - 1452 WP_048265233.1 PLP-dependent aminotransferase family protein -
HV227_RS21000 4163860..4164558 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
HV227_RS21005 4164555..4164695 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HV227_RS21010 4164695..4164958 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163471 WP_002892050.1 NZ_CP056425:4160787-4161005 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163471 NZ_CP072598:2731749-2731967 [Duffyella gerundensis]
ATGAGTGACAAAACACTGACTAAAACTGATTACCTGATGCGCTTAAGACGTTGCCGATCTATCGATACCCTTGAGCGTGT
TATTGAAAAAAACAAATATGAATTACCCGATAATGAACTGGCCATATTTTATTCAGCTGCCGATCATCGTTTAGCAGAAC
TGACGATGAATAAGCTTTACGATAAAGTGCCGGTTTCTGTCTGGAAATTTGTACGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT163471 WP_002892066.1 NZ_CP056425:4160386-4160760 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT163471 NZ_CP072598:2731342-2731716 [Duffyella gerundensis]
ATGGACGAATACTCACCTAAAAGGCATGATATGGCGCAGCTTAAGTACCTGTGCGAAAGTCTGCATGACGATAGCATGGC
CACCTTAAGTGACAGCCATCACGGCTGGGTTAATGATCCAACCTCTGCCAGCAATTTGCAGCTCAATGACCTGATTGAAC
ATATCGCATCCTTCACCATGAATTACAAAATTAAACATGTTGAAGATGAAGAACTGATTACTCAGATTGATGAATATCTT
GACGATACTTTTATGTTATTTAGTAACTATGGCGTAAGTGCGCAAGATCTGCAGCGTTGGCAACGTTCGGCAAAGCGGCT
TTTTAATATATTTGCCGAGGAATGCGCTCTGTTACAACCGAGCCATTCATTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References