Detailed information of TA system TA163457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 11057..11716
• Replicon: plasmid pRHBSTW-00524_3
• Accession: NZ_CP056423
• Organism: Citrobacter sp. RHBSTW-00524

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HV229_RS25865
• Protein ID: WP_119173804.1
• Coordinates: 11534..11716 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV229_RS25860
• Protein ID: WP_119173805.1
• Coordinates: 11057..11491 (-)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV229_RS25810	6590..6790	+	201	WP_181547816.1	hypothetical protein	-
HV229_RS25815	6793..7407	+	615	WP_181547818.1	3'-5' exonuclease	-
HV229_RS25820	7408..7656	+	249	WP_171845778.1	hypothetical protein	-
HV229_RS25825	7662..7892	+	231	WP_119173849.1	hypothetical protein	-
HV229_RS25830	7886..8188	+	303	WP_181547836.1	hypothetical protein	-
HV229_RS25835	8192..8341	+	150	Protein_10	DUF550 domain-containing protein	-
HV229_RS25840	8689..9129	+	441	WP_181547838.1	DUF550 domain-containing protein	-
HV229_RS25845	9126..10121	+	996	WP_181547820.1	hypothetical protein	-
HV229_RS25850	10121..10447	+	327	WP_171845780.1	hypothetical protein	-
HV229_RS25855	10468..10689	+	222	WP_119173806.1	hypothetical protein	-
HV229_RS25860	11057..11491	-	435	WP_119173805.1	hypothetical protein	Antitoxin
HV229_RS25865	11534..11716	-	183	WP_119173804.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV229_RS25870	11908..12129	+	222	WP_171845781.1	hypothetical protein	-
HV229_RS25875	12140..12367	+	228	WP_119173802.1	hypothetical protein	-
HV229_RS25880	12633..13703	+	1071	WP_171845782.1	site-specific DNA-methyltransferase	-
HV229_RS25885	13766..14077	+	312	WP_119173800.1	hypothetical protein	-
HV229_RS25890	14074..14562	+	489	WP_181547822.1	hypothetical protein	-
HV229_RS25895	14582..14941	+	360	WP_181547824.1	hypothetical protein	-
HV229_RS25900	14975..15187	+	213	WP_181547846.1	hypothetical protein	-
HV229_RS25905	15313..15618	+	306	WP_171845784.1	DUF4406 domain-containing protein	-
HV229_RS25910	15630..16640	+	1011	WP_181547826.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..47363	47363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-82)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6826.96 Da
• Isoelectric Point: 10.8173

>T163457 WP_119173804.1 NZ_CP056423:c11716-11534 [Citrobacter sp. RHBSTW-00524]
VKYSEFRRWLVQQGAEFKKAPGGGSHQKVSLNGMRSIFPDHGSKEMPEPLRKKIMKDLGL

Nucleotide

• Length: 183 bp

>T163457 NZ_CP072581:25774-26031 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 15743.98 Da
• Isoelectric Point: 4.8085

>AT163457 WP_119173805.1 NZ_CP056423:c11491-11057 [Citrobacter sp. RHBSTW-00524]
MFNYPVKLEYDTDTGAYIVSCRDLPLMNSVGYSVEEALLEASYGLVAAVAIEIEERRPVPQGSEPQEGEYVVYLPVLPAM
KAALHNAMIETGTRKAELARKLGKSGTQIDRLLDVEHSSKAETIELALHQLNKNIGVSVAAARQ

Nucleotide

• Length: 435 bp

>AT163457 NZ_CP072581:25512-25781 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA