Detailed information of TA system TA163456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 37868..38665
• Replicon: plasmid pRHBSTW-00524_2
• Accession: NZ_CP056422
• Organism: Citrobacter sp. RHBSTW-00524

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV229_RS25370
• Protein ID: WP_172754447.1
• Coordinates: 38162..38665 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: HV229_RS25365
• Protein ID: WP_016582626.1
• Coordinates: 37868..38137 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV229_RS25345	33754..34479	-	726	WP_130715040.1	hypothetical protein	-
HV229_RS25350	34671..36374	-	1704	WP_131405960.1	hypothetical protein	-
HV229_RS25355	36423..36776	-	354	WP_160859104.1	hypothetical protein	-
HV229_RS25360	36782..37447	-	666	WP_023315962.1	AAA family ATPase	-
HV229_RS25365	37868..38137	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
HV229_RS25370	38162..38665	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
HV229_RS25375	38693..38899	-	207	WP_023316023.1	hypothetical protein	-
HV229_RS25380	38850..39101	-	252	WP_000147960.1	hypothetical protein	-
HV229_RS25385	39103..39795	-	693	WP_006812500.1	membrane protein	-
HV229_RS25390	39809..40132	-	324	WP_000064173.1	hypothetical protein	-
HV229_RS25395	40207..40995	-	789	WP_181547782.1	receptor-recognizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..112583	112583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T163456 WP_172754447.1 NZ_CP056422:38162-38665 [Citrobacter sp. RHBSTW-00524]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T163456 NZ_CP072580:c220840-220568 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT163456 WP_016582626.1 NZ_CP056422:37868-38137 [Citrobacter sp. RHBSTW-00524]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT163456 NZ_CP072580:c221103-220840 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3