Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4135659..4136338 Replicon chromosome
Accession NZ_CP056421
Organism Citrobacter sp. RHBSTW-00524

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2I8S2T2
Locus tag HV229_RS19965 Protein ID WP_008324514.1
Coordinates 4135997..4136338 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A2I8S2U6
Locus tag HV229_RS19960 Protein ID WP_008324516.1
Coordinates 4135659..4135976 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV229_RS19915 4131097..4131918 + 822 WP_001547762.1 DUF945 domain-containing protein -
HV229_RS19920 4132135..4132836 + 702 WP_001581462.1 WYL domain-containing protein -
HV229_RS19925 4132877..4133113 + 237 WP_001144031.1 hypothetical protein -
HV229_RS19930 4133113..4133556 + 444 WP_001547764.1 phage transcriptional regulator -
HV229_RS19935 4133579..4134046 + 468 WP_181546219.1 hypothetical protein -
HV229_RS19940 4134123..4134362 + 240 WP_181546221.1 DUF905 domain-containing protein -
HV229_RS19945 4134460..4134918 + 459 WP_000211838.1 antirestriction protein -
HV229_RS19950 4134934..4135410 + 477 WP_000811693.1 RadC family protein -
HV229_RS19955 4135419..4135646 + 228 WP_008324517.1 DUF987 domain-containing protein -
HV229_RS19960 4135659..4135976 + 318 WP_008324516.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV229_RS19965 4135997..4136338 + 342 WP_008324514.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV229_RS19975 4136961..4137425 - 465 WP_137352773.1 isoprenylcysteine carboxylmethyltransferase family protein -
HV229_RS19980 4137619..4139067 - 1449 WP_181546223.1 EAL domain-containing protein -
HV229_RS19985 4139908..4140605 + 698 Protein_3924 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4109171..4141366 32195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12919.98 Da        Isoelectric Point: 9.6543

>T163453 WP_008324514.1 NZ_CP056421:4135997-4136338 [Citrobacter sp. RHBSTW-00524]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T163453 NZ_CP072579:183541-183807 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11787.33 Da        Isoelectric Point: 7.3476

>AT163453 WP_008324516.1 NZ_CP056421:4135659-4135976 [Citrobacter sp. RHBSTW-00524]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT163453 NZ_CP072579:183278-183541 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S2T2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S2U6

References