Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3879146..3879766 Replicon chromosome
Accession NZ_CP056421
Organism Citrobacter sp. RHBSTW-00524

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV229_RS18730 Protein ID WP_002892050.1
Coordinates 3879548..3879766 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV229_RS18725 Protein ID WP_003021733.1
Coordinates 3879146..3879520 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV229_RS18715 3874293..3875486 + 1194 WP_181546135.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV229_RS18720 3875509..3878658 + 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV229_RS18725 3879146..3879520 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV229_RS18730 3879548..3879766 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV229_RS18735 3879947..3880498 + 552 WP_181546137.1 maltose O-acetyltransferase -
HV229_RS18740 3880615..3881085 + 471 WP_016152071.1 YlaC family protein -
HV229_RS18745 3881164..3881304 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV229_RS18750 3881306..3881566 - 261 WP_016152070.1 type B 50S ribosomal protein L31 -
HV229_RS18755 3881755..3883308 + 1554 WP_053388979.1 EAL domain-containing protein -
HV229_RS18760 3883360..3883713 - 354 WP_003831033.1 DUF1428 family protein -
HV229_RS18765 3883778..3884407 - 630 WP_016155872.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163452 WP_002892050.1 NZ_CP056421:3879548-3879766 [Citrobacter sp. RHBSTW-00524]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163452 NZ_CP072569:32560-32817 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163452 WP_003021733.1 NZ_CP056421:3879146-3879520 [Citrobacter sp. RHBSTW-00524]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163452 NZ_CP072569:32298-32567 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References