Detailed information of TA system TA163441

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1591518..1592197
• Replicon: chromosome
• Accession: NZ_CP056421
• Organism: Citrobacter sp. RHBSTW-00524

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV229_RS07600
• Protein ID: WP_114263376.1
• Coordinates: 1591518..1591859 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV229_RS07605
• Protein ID: WP_115615054.1
• Coordinates: 1591880..1592197 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV229_RS07585	1586633..1589707	-	3075	WP_040119782.1	beta-galactosidase	-
HV229_RS07590	1589830..1590912	-	1083	WP_069729011.1	DNA-binding transcriptional repressor LacI	-
HV229_RS07595	1591149..1591370	-	222	Protein_1482	transposase	-
HV229_RS07600	1591518..1591859	-	342	WP_114263376.1	TA system toxin CbtA family protein	Toxin
HV229_RS07605	1591880..1592197	-	318	WP_115615054.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV229_RS07610	1592215..1592436	-	222	WP_181547178.1	DUF987 domain-containing protein	-
HV229_RS07615	1592445..1592927	-	483	WP_181547180.1	DNA repair protein RadC	-
HV229_RS07620	1592936..1593400	-	465	WP_181547182.1	antirestriction protein	-
HV229_RS07625	1593542..1596385	-	2844	WP_181547184.1	Ag43/Cah family autotransporter adhesin	-
HV229_RS07630	1596458..1597144	-	687	WP_047738081.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1571372..1598198	26826
	-	flank	IS/Tn	-	-	1591080..1591370	290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12773.84 Da
• Isoelectric Point: 6.9769

>T163441 WP_114263376.1 NZ_CP056421:c1591859-1591518 [Citrobacter sp. RHBSTW-00524]
MKTLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEYIDAGISLANAVNFLVEKYELVRIDRRGF
SWQEQSPYLLAVDILQARQAIGLLRRSLNDAVL

Nucleotide

• Length: 342 bp

>T163441 NZ_CP072566:2613787-2613894 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11884.48 Da
• Isoelectric Point: 6.6249

>AT163441 WP_115615054.1 NZ_CP056421:c1592197-1591880 [Citrobacter sp. RHBSTW-00524]
MSNTTWGLQRDITPRLEARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT163441 NZ_CP072566:c2613740-2613674 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT