Detailed information of TA system TA163439

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 948580..949234
• Replicon: chromosome
• Accession: NZ_CP056421
• Organism: Citrobacter sp. RHBSTW-00524

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV229_RS04675
• Protein ID: WP_016154348.1
• Coordinates: 948827..949234 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV229_RS04670
• Protein ID: WP_016154349.1
• Coordinates: 948580..948846 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV229_RS04645	943793..945226	-	1434	WP_181546972.1	6-phospho-beta-glucosidase BglA	-
HV229_RS04650	945347..946075	-	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
HV229_RS04655	946128..946439	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV229_RS04660	946603..947262	+	660	WP_016154351.1	hemolysin III family protein	-
HV229_RS04665	947342..948322	-	981	WP_181546974.1	tRNA-modifying protein YgfZ	-
HV229_RS04670	948580..948846	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV229_RS04675	948827..949234	+	408	WP_016154348.1	protein YgfX	Toxin
HV229_RS04680	949335..949856	-	522	WP_016154347.1	flavodoxin FldB	-
HV229_RS04685	949970..950866	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV229_RS04690	950890..951603	+	714	WP_181546976.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV229_RS04695	951609..953342	+	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T163439 WP_016154348.1 NZ_CP056421:948827-949234 [Citrobacter sp. RHBSTW-00524]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T163439 NZ_CP072566:c2425690-2425514 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT163439 WP_016154349.1 NZ_CP056421:948580-948846 [Citrobacter sp. RHBSTW-00524]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT163439 NZ_CP072566:c2425468-2425052 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6