Detailed information of TA system TA163435

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4190627..4191466
• Replicon: chromosome
• Accession: NZ_CP056409
• Organism: Citrobacter sp. RHBSTW-00535

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HV232_RS20015
• Protein ID: WP_094169374.1
• Coordinates: 4191089..4191466 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV232_RS20010
• Protein ID: WP_077249796.1
• Coordinates: 4190627..4191040 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV232_RS19965	4185718..4186605	+	888	WP_000682561.1	50S ribosome-binding GTPase	-
HV232_RS19970	4186811..4187212	+	402	WP_000819262.1	hypothetical protein	-
HV232_RS19975	4187309..4187776	+	468	WP_000727137.1	hypothetical protein	-
HV232_RS19980	4187967..4188217	+	251	Protein_3914	DUF905 family protein	-
HV232_RS19985	4188333..4189151	+	819	WP_094169373.1	DUF945 domain-containing protein	-
HV232_RS19990	4189151..4189414	+	264	WP_038633754.1	hypothetical protein	-
HV232_RS19995	4189492..4189887	+	396	WP_176443286.1	antirestriction protein	-
HV232_RS20000	4189898..4190425	+	528	WP_077249797.1	DNA repair protein RadC	-
HV232_RS20005	4190437..4190658	+	222	WP_038633763.1	DUF987 domain-containing protein	-
HV232_RS20010	4190627..4191040	+	414	WP_077249796.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV232_RS20015	4191089..4191466	+	378	WP_094169374.1	TA system toxin CbtA family protein	Toxin
HV232_RS20020	4191463..4191954	+	492	WP_040063379.1	hypothetical protein	-
HV232_RS20025	4191984..4192178	+	195	WP_038633777.1	DUF957 domain-containing protein	-
HV232_RS20030	4192259..4193107	+	849	WP_040229927.1	DUF4942 domain-containing protein	-
HV232_RS20035	4193868..4194194	+	327	Protein_3925	PAAR domain-containing protein	-
HV232_RS20040	4194496..4195659	+	1164	WP_094169375.1	S-type pyocin domain-containing protein	-
HV232_RS20045	4195656..4196123	+	468	WP_094169376.1	pyocin immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4181379..4234246	52867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (33-132)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14038.90 Da
• Isoelectric Point: 6.8604

>T163435 WP_094169374.1 NZ_CP056409:4191089-4191466 [Citrobacter sp. RHBSTW-00535]
MQTQPVSPAREASPRMSPVEIWQRLLSHLLDHHYGLALNDTPFGNDDVIQEHIDAGISLCDAVNFIVEKYDLVRTDRYGS
STTEQSPLISSIDILRARKACGLMTRNGYRAVTDITTGKYSKMTR

Nucleotide

• Length: 378 bp

>T163435 NZ_CP072566:1813544-1813798 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15178.32 Da
• Isoelectric Point: 7.7885

>AT163435 WP_077249796.1 NZ_CP056409:4190627-4191040 [Citrobacter sp. RHBSTW-00535]
MSFCAASPSVNSYSRSTTLLNKTLPANHHISEPWWGLKRNITPCFGARLVQEGNRLHYLADRASITGTFTDADLRHLDQA
FPVLLKQMELMLVSGELSPRHQHCVTLYAKGLTCEADSLGSHGYIYIAIYPTPSVTS

Nucleotide

• Length: 414 bp

>AT163435 NZ_CP072566:1813296-1813547 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA