Detailed information of TA system TA163434

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4116638..4117391
• Replicon: chromosome
• Accession: NZ_CP056409
• Organism: Citrobacter sp. RHBSTW-00535

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: HV232_RS19640
• Protein ID: WP_016154457.1
• Coordinates: 4117014..4117391 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV232_RS19635
• Protein ID: WP_162838704.1
• Coordinates: 4116638..4116964 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV232_RS19575	4111697..4111963	+	267	WP_032950091.1	hypothetical protein	-
HV232_RS19580	4111976..4112287	+	312	WP_009652410.1	hypothetical protein	-
HV232_RS19585	4112334..4112804	+	471	WP_009652431.1	hypothetical protein	-
HV232_RS19590	4112853..4113365	+	513	WP_032950094.1	DUF4234 domain-containing protein	-
HV232_RS19595	4113447..4113668	+	222	WP_032950095.1	hypothetical protein	-
HV232_RS19600	4113724..4113957	+	234	WP_009652474.1	DUF905 domain-containing protein	-
HV232_RS19605	4114061..4114879	+	819	WP_009652424.1	DUF945 domain-containing protein	-
HV232_RS19610	4114879..4115133	+	255	WP_009652519.1	hypothetical protein	-
HV232_RS19615	4115162..4115623	+	462	WP_045890390.1	antirestriction protein	-
HV232_RS19620	4115634..4115852	+	219	WP_009652503.1	hypothetical protein	-
HV232_RS19625	4115864..4116346	+	483	WP_016154455.1	DNA repair protein RadC	-
HV232_RS19630	4116360..4116581	+	222	WP_009652392.1	DUF987 domain-containing protein	-
HV232_RS19635	4116638..4116964	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV232_RS19640	4117014..4117391	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
HV232_RS19645	4117388..4117879	+	492	WP_009652438.1	hypothetical protein	-
HV232_RS19650	4117909..4118112	+	204	WP_009652505.1	DUF957 domain-containing protein	-
HV232_RS19655	4118193..4119038	+	846	Protein_3849	DUF4942 domain-containing protein	-
HV232_RS19660	4119474..4120727	+	1254	WP_040232077.1	integrase arm-type DNA-binding domain-containing protein	-
HV232_RS19665	4120934..4121995	+	1062	WP_040232076.1	nucleoid-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T163434 WP_016154457.1 NZ_CP056409:4117014-4117391 [Citrobacter sp. RHBSTW-00535]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T163434 NZ_CP072566:1813296-1813547 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT163434 WP_162838704.1 NZ_CP056409:4116638-4116964 [Citrobacter sp. RHBSTW-00535]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT163434 NZ_CP072566:1813544-1813798 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: R8WN26