Detailed information of TA system TA163433

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3949537..3950191
• Replicon: chromosome
• Accession: NZ_CP056409
• Organism: Citrobacter sp. RHBSTW-00535

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HV232_RS18795
• Protein ID: WP_094168630.1
• Coordinates: 3949537..3949944 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV232_RS18800
• Protein ID: WP_016154349.1
• Coordinates: 3949925..3950191 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV232_RS18775	3945429..3947162	-	1734	WP_016154344.1	single-stranded-DNA-specific exonuclease RecJ	-
HV232_RS18780	3947168..3947881	-	714	WP_047357820.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV232_RS18785	3947905..3948801	-	897	WP_046274931.1	site-specific tyrosine recombinase XerD	-
HV232_RS18790	3948915..3949436	+	522	WP_016154347.1	flavodoxin FldB	-
HV232_RS18795	3949537..3949944	-	408	WP_094168630.1	protein YgfX	Toxin
HV232_RS18800	3949925..3950191	-	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV232_RS18805	3950449..3951429	+	981	WP_094168631.1	tRNA-modifying protein YgfZ	-
HV232_RS18810	3951509..3952168	-	660	WP_016154351.1	hemolysin III family protein	-
HV232_RS18815	3952245..3953261	+	1017	WP_094169092.1	IS5-like element IS5 family transposase	-
HV232_RS18820	3953531..3953842	-	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV232_RS18825	3953895..3954623	+	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3952281..3953261	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15864.69 Da
• Isoelectric Point: 11.2845

>T163433 WP_094168630.1 NZ_CP056409:c3949944-3949537 [Citrobacter sp. RHBSTW-00535]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMIKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T163433 NZ_CP072566:1018530-1018865 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT163433 WP_016154349.1 NZ_CP056409:c3950191-3949925 [Citrobacter sp. RHBSTW-00535]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT163433 NZ_CP072566:1018282-1018530 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: R8WMJ6