Detailed information of TA system TA163432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2518851..2519487
• Replicon: chromosome
• Accession: NZ_CP056409
• Organism: Citrobacter sp. RHBSTW-00535

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV232_RS12095
• Protein ID: WP_049259794.1
• Coordinates: 2519299..2519487 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV232_RS12090
• Protein ID: WP_131341011.1
• Coordinates: 2518851..2519267 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV232_RS12070	2513896..2514840	-	945	WP_016153152.1	ABC transporter permease	-
HV232_RS12075	2514841..2515857	-	1017	WP_016156582.1	ABC transporter ATP-binding protein	-
HV232_RS12080	2515874..2517019	-	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV232_RS12085	2517349..2518758	-	1410	WP_019076828.1	PLP-dependent aminotransferase family protein	-
HV232_RS12090	2518851..2519267	-	417	WP_131341011.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV232_RS12095	2519299..2519487	-	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV232_RS12100	2519751..2521154	+	1404	WP_047417372.1	cytochrome ubiquinol oxidase subunit I	-
HV232_RS12105	2521154..2522164	+	1011	WP_016153157.1	cytochrome d ubiquinol oxidase subunit II	-
HV232_RS12110	2522164..2522289	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV232_RS12115	2522479..2522709	+	231	WP_016153158.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T163432 WP_049259794.1 NZ_CP056409:c2519487-2519299 [Citrobacter sp. RHBSTW-00535]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T163432 NZ_CP072566:884573-884980 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15034.31 Da
• Isoelectric Point: 4.7119

>AT163432 WP_131341011.1 NZ_CP056409:c2519267-2518851 [Citrobacter sp. RHBSTW-00535]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGAEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT163432 NZ_CP072566:884326-884592 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9