Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1311737..1312357 Replicon chromosome
Accession NZ_CP056409
Organism Citrobacter sp. RHBSTW-00535

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV232_RS06280 Protein ID WP_002892050.1
Coordinates 1311737..1311955 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV232_RS06285 Protein ID WP_003021733.1
Coordinates 1311983..1312357 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV232_RS06245 1307096..1307725 + 630 WP_016155872.1 membrane protein -
HV232_RS06250 1307790..1308143 + 354 WP_003831033.1 DUF1428 family protein -
HV232_RS06255 1308195..1309748 - 1554 WP_047358017.1 EAL domain-containing protein -
HV232_RS06260 1309937..1310197 + 261 WP_016152070.1 type B 50S ribosomal protein L31 -
HV232_RS06265 1310199..1310339 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV232_RS06270 1310418..1310888 - 471 WP_016152071.1 YlaC family protein -
HV232_RS06275 1311005..1311556 - 552 WP_016152072.1 maltose O-acetyltransferase -
HV232_RS06280 1311737..1311955 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV232_RS06285 1311983..1312357 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV232_RS06290 1312845..1315994 - 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV232_RS06295 1316017..1317210 - 1194 WP_016152074.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163423 WP_002892050.1 NZ_CP056409:c1311955-1311737 [Citrobacter sp. RHBSTW-00535]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163423 NZ_CP072565:3438189-3438407 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163423 WP_003021733.1 NZ_CP056409:c1312357-1311983 [Citrobacter sp. RHBSTW-00535]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163423 NZ_CP072565:3437789-3438163 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References