Detailed information of TA system TA163422

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 687876..688597
• Replicon: chromosome
• Accession: NZ_CP056409
• Organism: Citrobacter sp. RHBSTW-00535

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV232_RS03370
• Protein ID: WP_047399350.1
• Coordinates: 688256..688597 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV232_RS03365
• Protein ID: WP_032676674.1
• Coordinates: 687876..688220 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV232_RS03335	684242..684676	+	435	WP_032676669.1	hypothetical protein	-
HV232_RS03340	684696..685499	+	804	WP_032676670.1	hypothetical protein	-
HV232_RS03345	685731..686186	+	456	WP_032676671.1	hypothetical protein	-
HV232_RS03350	686255..687076	+	822	WP_032676672.1	DUF945 domain-containing protein	-
HV232_RS03355	687147..687620	+	474	WP_057064709.1	DNA repair protein RadC	-
HV232_RS03360	687631..687852	+	222	WP_045265422.1	DUF987 family protein	-
HV232_RS03365	687876..688220	+	345	WP_032676674.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV232_RS03370	688256..688597	+	342	WP_047399350.1	TA system toxin CbtA family protein	Toxin
HV232_RS03375	688712..689545	+	834	WP_032676675.1	DUF4942 domain-containing protein	-
HV232_RS03380	689622..689870	+	249	WP_000535212.1	ribbon-helix-helix domain-containing protein	-
HV232_RS03385	689874..690149	+	276	WP_032676676.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV232_RS03390	690269..691066	+	798	WP_032676677.1	helix-turn-helix transcriptional regulator	-
HV232_RS03395	691226..692098	-	873	WP_081304345.1	HNH endonuclease	-
HV232_RS03400	692357..692806	+	450	WP_094169251.1	hypothetical protein	-
HV232_RS03405	692800..693126	+	327	WP_016151544.1	DUF1493 family protein	-
HV232_RS03410	693145..693261	+	117	Protein_668	type VI secretion system tube protein Hcp	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	666542..694390	27848
	-	flank	IS/Tn	-	-	693410..694390	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (14-113)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12748.67 Da
• Isoelectric Point: 8.5196

>T163422 WP_047399350.1 NZ_CP056409:688256-688597 [Citrobacter sp. RHBSTW-00535]
MNTSPATNQRVAKPCPSPVAVWQMLLTRLLAQHYGLALSDTPFCDETVIQEHINAGVTLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQAMGLLRQGHNLSTR

Nucleotide

• Length: 342 bp

>T163422 NZ_CP072565:2677880-2677987 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12877.92 Da
• Isoelectric Point: 8.1459

>AT163422 WP_032676674.1 NZ_CP056409:687876-688220 [Citrobacter sp. RHBSTW-00535]
MKKDTANEVVDTLRWGLKCDITPHFGARLVQEGNRLHYLADRASIIGPFSKADLRHLNQAFPLLMKQMELMLTSGELNPR
HQHCVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 345 bp

>AT163422 NZ_CP072565:c2677830-2677767 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT