Detailed information of TA system TA163420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 49698..50495
• Replicon: plasmid pRHBSTW-00570_3
• Accession: NZ_CP056401
• Organism: Citrobacter sp. RHBSTW-00570

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV237_RS25390
• Protein ID: WP_172754447.1
• Coordinates: 49992..50495 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: HV237_RS25385
• Protein ID: WP_016582626.1
• Coordinates: 49698..49967 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV237_RS25365	45584..46309	-	726	WP_130715040.1	hypothetical protein	-
HV237_RS25370	46501..48204	-	1704	WP_131405960.1	hypothetical protein	-
HV237_RS25375	48253..48606	-	354	WP_160859104.1	hypothetical protein	-
HV237_RS25380	48612..49277	-	666	WP_023315962.1	AAA family ATPase	-
HV237_RS25385	49698..49967	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
HV237_RS25390	49992..50495	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
HV237_RS25395	50524..50730	-	207	WP_165501435.1	hypothetical protein	-
HV237_RS25400	50681..50932	-	252	WP_000147960.1	hypothetical protein	-
HV237_RS25405	50934..51626	-	693	WP_006812500.1	membrane protein	-
HV237_RS25410	51640..51963	-	324	WP_000064173.1	hypothetical protein	-
HV237_RS25415	52038..52826	-	789	WP_110921389.1	receptor-recognizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..119726	119726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T163420 WP_172754447.1 NZ_CP056401:49992-50495 [Citrobacter sp. RHBSTW-00570]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T163420 NZ_CP072565:2676810-2676917 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT163420 WP_016582626.1 NZ_CP056401:49698-49967 [Citrobacter sp. RHBSTW-00570]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT163420 NZ_CP072565:c2676763-2676697 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3