Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 109681..110484 Replicon plasmid pRHBSTW-00570_2
Accession NZ_CP056400
Organism Citrobacter sp. RHBSTW-00570

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag HV237_RS24750 Protein ID WP_022652313.1
Coordinates 109954..110484 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag HV237_RS24745 Protein ID WP_022652312.1
Coordinates 109681..109950 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV237_RS24730 105986..106293 - 308 Protein_107 IS1 family transposase -
HV237_RS24735 106770..107510 + 741 WP_022652310.1 site-specific integrase -
HV237_RS24740 107836..108825 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
HV237_RS24745 109681..109950 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
HV237_RS24750 109954..110484 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
HV237_RS24755 110616..111509 + 894 WP_161348953.1 IS5 family transposase -
HV237_RS24760 111650..112126 - 477 Protein_113 IS3 family transposase -
HV237_RS24765 112207..113715 - 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
HV237_RS24770 114388..114817 - 430 Protein_115 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..179403 179403
- inside IScluster/Tn - - 89947..117878 27931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T163419 WP_022652313.1 NZ_CP056400:109954-110484 [Citrobacter sp. RHBSTW-00570]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T163419 NZ_CP072565:c2489248-2489072 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT163419 WP_022652312.1 NZ_CP056400:109681-109950 [Citrobacter sp. RHBSTW-00570]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT163419 NZ_CP072565:c2489026-2488610 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References