Detailed information of TA system TA163417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4916102..4916718
• Replicon: chromosome
• Accession: NZ_CP056399
• Organism: Citrobacter sp. RHBSTW-00570

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV237_RS23295
• Protein ID: WP_016155182.1
• Coordinates: 4916102..4916476 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R8WIS4
• Locus tag: HV237_RS23300
• Protein ID: WP_016155181.1
• Coordinates: 4916476..4916718 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV237_RS23280	4913605..4914507	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HV237_RS23285	4914504..4915139	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV237_RS23290	4915136..4916065	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV237_RS23295	4916102..4916476	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV237_RS23300	4916476..4916718	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV237_RS23305	4916924..4917853	+	930	WP_047357779.1	alpha/beta hydrolase	-
HV237_RS23310	4917938..4918249	+	312	WP_047357780.1	type II toxin-antitoxin system HigB family toxin	-
HV237_RS23315	4918246..4918698	+	453	WP_047357781.1	helix-turn-helix domain-containing protein	-
HV237_RS23320	4918716..4919657	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV237_RS23325	4919702..4920139	-	438	WP_016157874.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV237_RS23330	4920136..4921008	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HV237_RS23335	4921002..4921601	-	600	WP_016157873.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13678.84 Da
• Isoelectric Point: 8.5373

>T163417 WP_016155182.1 NZ_CP056399:c4916476-4916102 [Citrobacter sp. RHBSTW-00570]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T163417 NZ_CP072565:2332770-2333057 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTAAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.72 Da
• Isoelectric Point: 4.3331

>AT163417 WP_016155181.1 NZ_CP056399:c4916718-4916476 [Citrobacter sp. RHBSTW-00570]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT163417 NZ_CP072565:2332531-2332770 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: R8WIS4