Detailed information of TA system TA163415

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4384548..4385227
• Replicon: chromosome
• Accession: NZ_CP056399
• Organism: Citrobacter sp. RHBSTW-00570

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV237_RS20815
• Protein ID: WP_098992213.1
• Coordinates: 4384548..4384889 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV237_RS20820
• Protein ID: WP_116326589.1
• Coordinates: 4384910..4385227 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV237_RS20785	4380144..4380812	+	669	Protein_4075	HNH endonuclease	-
HV237_RS20790	4380895..4381278	+	384	WP_001201739.1	IS66 family insertion sequence hypothetical protein	-
HV237_RS20795	4381275..4381622	+	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-
HV237_RS20800	4381672..4383207	+	1536	WP_001000409.1	IS66 family transposase	-
HV237_RS20805	4383243..4383461	+	219	Protein_4079	HNH endonuclease	-
HV237_RS20810	4383792..4384433	+	642	WP_181541851.1	hypothetical protein	-
HV237_RS20815	4384548..4384889	-	342	WP_098992213.1	TA system toxin CbtA family protein	Toxin
HV237_RS20820	4384910..4385227	-	318	WP_116326589.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV237_RS20825	4385245..4385466	-	222	WP_098992211.1	DUF987 domain-containing protein	-
HV237_RS20830	4385475..4385951	-	477	WP_181541593.1	RadC family protein	-
HV237_RS20835	4385967..4386425	-	459	WP_181541595.1	antirestriction protein	-
HV237_RS20840	4386506..4386742	-	237	WP_181541597.1	DUF905 domain-containing protein	-
HV237_RS20845	4386820..4387230	-	411	WP_181541599.1	hypothetical protein	-
HV237_RS20850	4387320..4387934	-	615	WP_181541601.1	hypothetical protein	-
HV237_RS20855	4387931..4388635	-	705	WP_181541603.1	WYL domain-containing protein	-
HV237_RS20860	4388837..4389670	-	834	WP_181541605.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4379436..4405738	26302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12879.91 Da
• Isoelectric Point: 8.9786

>T163415 WP_098992213.1 NZ_CP056399:c4384889-4384548 [Citrobacter sp. RHBSTW-00570]
MKPQPATTSRAVKPCLSPVAVWQMLLTRLLEQHYGLKMNDTPFSDETVIKEHIDAGITLADAVNFLVDKYELVRIDRRGF
SWQEQSPYLRAVDILRARQATGLLRRSLNDAVL

Nucleotide

• Length: 342 bp

>T163415 NZ_CP072565:1877102-1877356 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11779.35 Da
• Isoelectric Point: 6.4753

>AT163415 WP_116326589.1 NZ_CP056399:c4385227-4384910 [Citrobacter sp. RHBSTW-00570]
MSNTTWGLQRDITPSLGARLVQEGNRLHYLADRASITGKFSDEECLKLDVVFPHFISQIESMLTTGELNPRHAHCVTLYH
NSFICEADTLGSCGYVHIAIYPAQR

Nucleotide

• Length: 318 bp

>AT163415 NZ_CP072565:1876854-1877105 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA