Detailed information of TA system TA163405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2492821..2493457
• Replicon: chromosome
• Accession: NZ_CP056399
• Organism: Citrobacter sp. RHBSTW-00570

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV237_RS11855
• Protein ID: WP_049259794.1
• Coordinates: 2492821..2493009 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV237_RS11860
• Protein ID: WP_131341011.1
• Coordinates: 2493041..2493457 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV237_RS11835	2489599..2489829	-	231	WP_016153158.1	DUF2554 family protein	-
HV237_RS11840	2490019..2490144	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV237_RS11845	2490144..2491154	-	1011	WP_016153157.1	cytochrome d ubiquinol oxidase subunit II	-
HV237_RS11850	2491154..2492557	-	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
HV237_RS11855	2492821..2493009	+	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV237_RS11860	2493041..2493457	+	417	WP_131341011.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV237_RS11865	2493550..2494959	+	1410	WP_047358125.1	PLP-dependent aminotransferase family protein	-
HV237_RS11870	2495289..2496434	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV237_RS11875	2496451..2497467	+	1017	WP_016156582.1	ABC transporter ATP-binding protein	-
HV237_RS11880	2497468..2498412	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T163405 WP_049259794.1 NZ_CP056399:2492821-2493009 [Citrobacter sp. RHBSTW-00570]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T163405 NZ_CP072564:3687808-3688149 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15034.31 Da
• Isoelectric Point: 4.7119

>AT163405 WP_131341011.1 NZ_CP056399:2493041-2493457 [Citrobacter sp. RHBSTW-00570]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGAEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT163405 NZ_CP072564:3687470-3687787 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9