Detailed information of TA system TA163403

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 990134..990906
• Replicon: chromosome
• Accession: NZ_CP056399
• Organism: Citrobacter sp. RHBSTW-00570

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ICB7
• Locus tag: HV237_RS04795
• Protein ID: WP_049238634.1
• Coordinates: 990517..990906 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV237_RS04790
• Protein ID: WP_171547612.1
• Coordinates: 990134..990460 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV237_RS04760	986527..987159	-	633	WP_181541740.1	inovirus Gp2 family protein	-
HV237_RS04765	987749..988573	+	825	WP_049238647.1	DUF945 domain-containing protein	-
HV237_RS04770	988570..988827	+	258	WP_049238645.1	DUF905 domain-containing protein	-
HV237_RS04775	988907..989350	+	444	WP_049238643.1	antirestriction protein	-
HV237_RS04780	989362..989841	+	480	WP_049238640.1	DNA repair protein RadC	-
HV237_RS04785	989855..990076	+	222	WP_049238638.1	DUF987 domain-containing protein	-
HV237_RS04790	990134..990460	+	327	WP_171547612.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV237_RS04795	990517..990906	+	390	WP_049238634.1	TA system toxin CbtA family protein	Toxin
HV237_RS04800	990993..991832	+	840	WP_181541742.1	DUF4942 domain-containing protein	-
HV237_RS04805	993246..993773	+	528	WP_046274927.1	Vi polysaccharide biosynthesis protein TviA	-
HV237_RS04810	994031..995308	+	1278	WP_016157409.1	Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	tviB / tviC	953498..996351	42853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14387.45 Da
• Isoelectric Point: 9.6254

>T163403 WP_049238634.1 NZ_CP056399:990517-990906 [Citrobacter sp. RHBSTW-00570]
MQTKLSPLMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDEQVIQQHIDAGISLSDALNFIVEKYELVRTDRPGF
SIREQSPFITPIDILRARKAAGLMNRGTYKEVTAITRGQHPQANASGKR

Nucleotide

• Length: 390 bp

>T163403 NZ_CP072564:3438175-3438393 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12174.84 Da
• Isoelectric Point: 6.4673

>AT163403 WP_171547612.1 NZ_CP056399:990134-990460 [Citrobacter sp. RHBSTW-00570]
ISEPWWGLRRSVSPCFGARLVQERNSLHYLADRANFDGQFCDADLRYLDQAFPVLMKQLELMLTSGELNPRHQHGVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVTTA

Nucleotide

• Length: 327 bp

>AT163403 NZ_CP072564:3437775-3438149 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7L6ICB7