Detailed information of TA system TA163400

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 678820..679478
• Replicon: chromosome
• Accession: NZ_CP056399
• Organism: Citrobacter sp. RHBSTW-00570

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV237_RS03285
• Protein ID: WP_181541707.1
• Coordinates: 679158..679478 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV237_RS03280
• Protein ID: WP_181541706.1
• Coordinates: 678820..679137 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV237_RS03235	673941..674768	+	828	WP_181541692.1	DUF945 domain-containing protein	-
HV237_RS03240	674995..675702	+	708	WP_181541693.1	transcriptional regulator	-
HV237_RS03245	675728..676264	+	537	WP_181541695.1	DUF4339 domain-containing protein	-
HV237_RS03250	676306..676743	+	438	WP_050150434.1	hypothetical protein	-
HV237_RS03255	676810..677220	+	411	WP_181541697.1	hypothetical protein	-
HV237_RS03260	677298..677534	+	237	WP_181541699.1	DUF905 domain-containing protein	-
HV237_RS03265	677620..678078	+	459	WP_181541701.1	antirestriction protein	-
HV237_RS03270	678094..678570	+	477	WP_181541703.1	RadC family protein	-
HV237_RS03275	678579..678800	+	222	WP_181541705.1	DUF987 domain-containing protein	-
HV237_RS03280	678820..679137	+	318	WP_181541706.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV237_RS03285	679158..679478	+	321	WP_181541707.1	TA system toxin CbtA family protein	Toxin
HV237_RS03290	679893..680135	+	243	WP_153742334.1	DinI family protein	-
HV237_RS03295	680293..680715	+	423	WP_153742333.1	hypothetical protein	-
HV237_RS03300	680943..683069	+	2127	WP_181541709.1	TonB-dependent siderophore receptor	-
HV237_RS03305	683082..684092	+	1011	WP_181541711.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	652980..680715	27735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-104)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12201.18 Da
• Isoelectric Point: 8.0618

>T163400 WP_181541707.1 NZ_CP056399:679158-679478 [Citrobacter sp. RHBSTW-00570]
MKTLPATTQRAVKHCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPFITAVDILKARRAMREMRT

Nucleotide

• Length: 321 bp

>T163400 NZ_CP072564:2676796-2676903 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11985.73 Da
• Isoelectric Point: 7.4139

>AT163400 WP_181541706.1 NZ_CP056399:678820-679137 [Citrobacter sp. RHBSTW-00570]
MTHIIWGLQRAITPRLGARLVQEGNRMHYLADRASITGKFSDAECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NGFTCEADTLGSCGYIYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT163400 NZ_CP072564:c2676749-2676683 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT