Detailed information of TA system TA163386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1191707..1192327
• Replicon: chromosome
• Accession: NZ_CP056395
• Organism: Citrobacter freundii strain RHBSTW-00589

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV241_RS05645
• Protein ID: WP_002892050.1
• Coordinates: 1191707..1191925 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV241_RS05650
• Protein ID: WP_115259201.1
• Coordinates: 1191953..1192327 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV241_RS05615	1187537..1188148	+	612	WP_115259218.1	hypothetical protein	-
HV241_RS05620	1188231..1188584	+	354	WP_115259217.1	DUF1428 family protein	-
HV241_RS05625	1188638..1190191	-	1554	WP_181625055.1	EAL domain-containing protein	-
HV241_RS05630	1190376..1190636	+	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HV241_RS05635	1190638..1190778	+	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV241_RS05640	1190852..1191325	-	474	WP_181625056.1	YlaC family protein	-
HV241_RS05645	1191707..1191925	-	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV241_RS05650	1191953..1192327	-	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HV241_RS05655	1192816..1195965	-	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HV241_RS05660	1195988..1197181	-	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T163386 WP_002892050.1 NZ_CP056395:c1191925-1191707 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T163386 NZ_CP072561:c17955-17635 [Pseudomonas sp. BYT-1]
ATGCAGCGAGGCGACCTCGTGACGGTTTCATTGCAGGGTGACTACGGCAAACCACGACCAGCCCTGATCGTTCAATCAGA
CCTACTAACCGACTTGGAAAGCGTTGTGCTGTGCCCAGTGACAAGCGACCTGCGCAATGCCGCGTTTCGCGTCACCGTGG
AACCGAATCCAGCTAACGGGCTGCGCGCACTATCGCAAGTTATGGTGGACAAGCTCTCGACGCTACCGCGCACCAAAATC
AGTGAACCGTTTGGCCGCCTCGACGATGAGCGAATGAAAGCAATAGATAGGGCGCTCCTGTTGGTCGTTGGCGTTATTTG
A

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT163386 WP_115259201.1 NZ_CP056395:c1192327-1191953 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT163386 NZ_CP072561:c18170-17955 [Pseudomonas sp. BYT-1]
ATGCCATCAATTCACGAATCAAAGGTAGCCCGACACCGCGAACGGATGCGCGCGGCGGGGCTGCGGCCTGTGCAGTTTTG
GGTGCCGGACACCCGTTCGCCTGAGTTCACGGCGCAAGTGCGCAAGCAATGCCAAAGCCTGAAGGGCGACCCAGCAGAAG
CAGAGGTTTTGCGCTTCACCGAAGAGGCGGCATCGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2