Detailed information of TA system TA163385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 620790..621306
• Replicon: chromosome
• Accession: NZ_CP056395
• Organism: Citrobacter freundii strain RHBSTW-00589

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5I6CHB9
• Locus tag: HV241_RS03060
• Protein ID: WP_000220581.1
• Coordinates: 621022..621306 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HV241_RS03055
• Protein ID: WP_115259576.1
• Coordinates: 620790..621032 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV241_RS03035	615805..616938	+	1134	WP_181624946.1	amidohydrolase/deacetylase family metallohydrolase	-
HV241_RS03040	616922..618040	+	1119	WP_096758517.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
HV241_RS03045	618037..618777	+	741	WP_181624947.1	KDGP aldolase family protein	-
HV241_RS03050	618799..620712	+	1914	WP_181624948.1	BglG family transcription antiterminator	-
HV241_RS03055	620790..621032	+	243	WP_115259576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HV241_RS03060	621022..621306	+	285	WP_000220581.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV241_RS03065	621310..621774	-	465	WP_096759476.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HV241_RS03070	621896..624034	-	2139	WP_115259574.1	anaerobic ribonucleoside-triphosphate reductase	-
HV241_RS03075	624453..626105	-	1653	WP_181624949.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10868.70 Da
• Isoelectric Point: 10.0482

>T163385 WP_000220581.1 NZ_CP056395:621022-621306 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQLKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T163385 NZ_CP072559:2939487-2939864 [Pseudomonas sp. BYT-1]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8947.32 Da
• Isoelectric Point: 5.0490

>AT163385 WP_115259576.1 NZ_CP056395:620790-621032 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLDKLNITPTEAVRLLFQYVAETGRMPVKTVTLSDSEDALIQTVRERLSSPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT163385 NZ_CP072559:2939845-2940180 [Pseudomonas sp. BYT-1]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A5I6CHB9