Detailed information of TA system TA163381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 27839..28476
• Replicon: plasmid pRHBSTW-00593_2
• Accession: NZ_CP056394
• Organism: Enterobacter sp. RHBSTW-00593

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV242_RS23775
• Protein ID: WP_111969555.1
• Coordinates: 27839..28249 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HV242_RS23780
• Protein ID: WP_111969554.1
• Coordinates: 28246..28476 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV242_RS23755	23152..24213	+	1062	WP_181616955.1	hypothetical protein	-
HV242_RS23760	24470..25060	-	591	WP_111969558.1	LuxR family transcriptional regulator	-
HV242_RS23765	25421..25801	-	381	WP_111969557.1	helix-turn-helix domain-containing protein	-
HV242_RS23770	26463..27329	+	867	WP_111969556.1	winged helix-turn-helix domain-containing protein	-
HV242_RS23775	27839..28249	-	411	WP_111969555.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV242_RS23780	28246..28476	-	231	WP_111969554.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV242_RS23785	29091..29417	+	327	WP_111969553.1	hypothetical protein	-
HV242_RS23790	29451..29780	+	330	WP_111969552.1	hypothetical protein	-
HV242_RS23795	30474..30821	+	348	WP_111969613.1	hypothetical protein	-
HV242_RS23800	30818..31600	+	783	WP_181616952.1	site-specific integrase	-
HV242_RS23805	32054..32686	+	633	WP_111969550.1	ParA family protein	-
HV242_RS23810	32686..33048	+	363	WP_111969549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89835	89835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.89 Da
• Isoelectric Point: 5.7351

>T163381 WP_111969555.1 NZ_CP056394:c28249-27839 [Enterobacter sp. RHBSTW-00593]
VNRIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAELSQAAKESGPGTQNLADAFAARLDAILPWDRAAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVPEDWVK

Nucleotide

• Length: 411 bp

>T163381 NZ_CP072558:29591-30061 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8634.77 Da
• Isoelectric Point: 4.6017

>AT163381 WP_111969554.1 NZ_CP056394:c28476-28246 [Enterobacter sp. RHBSTW-00593]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQMGKADPEFMAEREDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT163381 NZ_CP072558:29151-29594 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA