Detailed information of TA system TA163370

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 532268..533023
• Replicon: chromosome
• Accession: NZ_CP056393
• Organism: Enterobacter sp. RHBSTW-00593

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HV242_RS02540
• Protein ID: WP_181616902.1
• Coordinates: 532703..533023 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV242_RS02535
• Protein ID: WP_181616901.1
• Coordinates: 532268..532666 (+)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV242_RS02485	527736..528143	+	408	WP_181616893.1	hypothetical protein	-
HV242_RS02490	528216..528443	+	228	WP_181616894.1	hypothetical protein	-
HV242_RS02495	528443..528688	+	246	WP_181616895.1	hypothetical protein	-
HV242_RS02500	528746..529582	+	837	WP_181616896.1	hypothetical protein	-
HV242_RS02505	529676..529909	+	234	Protein_482	DUF905 domain-containing protein	-
HV242_RS02510	530007..530825	+	819	WP_181616897.1	DUF945 domain-containing protein	-
HV242_RS02515	530825..531067	+	243	WP_181616898.1	hypothetical protein	-
HV242_RS02520	531096..531575	+	480	WP_181616899.1	antirestriction protein	-
HV242_RS02525	531587..532066	+	480	WP_181616900.1	DNA repair protein RadC	-
HV242_RS02530	532106..532299	+	194	Protein_487	DUF987 family protein	-
HV242_RS02535	532268..532666	+	399	WP_181616901.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV242_RS02540	532703..533023	+	321	WP_181616902.1	TA system toxin CbtA family protein	Toxin
HV242_RS02545	533136..533969	+	834	WP_181616903.1	DUF4942 domain-containing protein	-
HV242_RS02550	534016..534735	+	720	WP_181616904.1	hypothetical protein	-
HV242_RS02555	535023..535898	-	876	WP_050737553.1	HNH endonuclease	-
HV242_RS02560	536199..536651	+	453	WP_072126500.1	type I toxin-antitoxin system SymE family toxin	-
HV242_RS02565	536747..537493	-	747	WP_050737552.1	class I SAM-dependent methyltransferase	-
HV242_RS02570	537504..537761	-	258	WP_014168433.1	YjhX family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	510694..534735	24041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-105)

Antitoxin

• Antitoxin CbeA (32-130)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12148.77 Da
• Isoelectric Point: 5.9558

>T163370 WP_181616902.1 NZ_CP056393:532703-533023 [Enterobacter sp. RHBSTW-00593]
MHISSVPYTVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAVIQEHIEAGITLADAVNFLVERYELVRTDRKGF
TWQEQTPFLTATDILRARRATGLINT

Nucleotide

• Length: 321 bp

>T163370 NZ_CP072555:c1861975-1861691 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14756.67 Da
• Isoelectric Point: 6.4608

>AT163370 WP_181616901.1 NZ_CP056393:532268-532666 [Enterobacter sp. RHBSTW-00593]
MSYCAASRSTNDNLRSPKLSNKTPYDHDVSEPWWGLERDITPCFGARLVQEGNRLHYLADRAGLVGSFSPEQLQKLDKAF
PLFIDQLEAMLCSGELNPRQRHQVSIHLTGLTCIADTCGSCGYVYLTIYSGQ

Nucleotide

• Length: 399 bp

>AT163370 NZ_CP072555:c1862207-1861965 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA