Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23235..23878 Replicon plasmid pRHBSTW-00610_2
Accession NZ_CP056386
Organism Klebsiella pneumoniae strain RHBSTW-00610

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HV247_RS26040 Protein ID WP_001044770.1
Coordinates 23462..23878 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HV247_RS26035 Protein ID WP_001261282.1
Coordinates 23235..23465 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV247_RS26005 19355..19990 + 636 WP_009309912.1 CPBP family intramembrane metalloprotease -
HV247_RS26010 20098..20442 - 345 WP_009309911.1 hypothetical protein -
HV247_RS26015 20764..21700 + 937 Protein_25 CAAX protease -
HV247_RS26020 21891..22331 - 441 WP_047057603.1 hypothetical protein -
HV247_RS26025 22350..22943 - 594 WP_009309909.1 hypothetical protein -
HV247_RS26030 23042..23278 - 237 Protein_28 hypothetical protein -
HV247_RS26035 23235..23465 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV247_RS26040 23462..23878 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HV247_RS26045 23952..25514 + 1563 WP_009309907.1 AAA family ATPase -
HV247_RS26050 25499..26521 + 1023 WP_000361404.1 helicase UvrD -
HV247_RS26055 27065..27973 + 909 WP_032425603.1 HNH endonuclease -
HV247_RS26060 28159..28509 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..252882 252882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T163366 WP_001044770.1 NZ_CP056386:23462-23878 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T163366 NZ_CP072552:67379-67699 [Acinetobacter lwoffii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTAAGTGATGAGGAATATCGTCAACTCCAGCA
AGATCTCTTGGTACAACCTGATAAAGGTGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAAGGCA
ATAAAGGTAAAAGTGGCGGTATCCGGGTGATCTATTATTGGGTCACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCTATTCTGCGTCAATTAGTGAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT163366 WP_001261282.1 NZ_CP056386:23235-23465 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT163366 NZ_CP072552:67692-67964 [Acinetobacter lwoffii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAACGTAATGAAATGAAAGCAAG
CCGAGTAACAGAGCTTGAATTGCCGGATATTAAAGAAGTACGTGAGAAAACTGGCTTGACCCAAGCTGAATTTGCTGCAC
GTTTGCATATCAGTGCCAGAACTCTGCAAAACTGGGAACAAGGCCGTCGTTATCCAACTGGTCCTGCAGCGACTTTAATT
CGTATTCTTGATGCTCATCCAAGTCTAATTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References