Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 13756..14399 Replicon plasmid pRHBSTW-00610_2
Accession NZ_CP056386
Organism Klebsiella pneumoniae strain RHBSTW-00610

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag HV247_RS25985 Protein ID WP_020804312.1
Coordinates 13983..14399 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HV247_RS25980 Protein ID WP_001261276.1
Coordinates 13756..13986 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV247_RS25940 9296..9409 + 114 WP_014343462.1 hypothetical protein -
HV247_RS25945 9538..9795 + 258 WP_009310077.1 hypothetical protein -
HV247_RS25950 9853..10632 - 780 WP_023287113.1 site-specific integrase -
HV247_RS25955 10830..11846 - 1017 WP_009309921.1 hypothetical protein -
HV247_RS25960 11880..12215 - 336 WP_009309920.1 hypothetical protein -
HV247_RS25965 12265..12405 - 141 WP_162898808.1 hypothetical protein -
HV247_RS25970 12634..12939 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
HV247_RS25975 12941..13159 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
HV247_RS25980 13756..13986 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV247_RS25985 13983..14399 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
HV247_RS25990 14440..15318 - 879 WP_032427228.1 restriction endonuclease -
HV247_RS25995 15980..17257 + 1278 WP_009309915.1 HlyD family secretion protein -
HV247_RS26000 17247..19355 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..252882 252882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T163365 WP_020804312.1 NZ_CP056386:13983-14399 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T163365 NZ_CP072552:c4509-4153 [Acinetobacter lwoffii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTGGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCATTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAACGATTCTATACAGAGATGCTGGCAATTGCAGACCAACAATATGAACT
TCATCTCTCAACATTAGGAGAACAATCTAATGGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT163365 WP_001261276.1 NZ_CP056386:13756-13986 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT163365 NZ_CP072552:c4160-3858 [Acinetobacter lwoffii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTAGAAATTTCTCAAAAAGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAAGCGATG
GGTGGAAAGCTCCGTATTGATGTAGAGCTTCCGACAGGAAAACATATAGGATTCAATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References