Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 12634..13159 Replicon plasmid pRHBSTW-00610_2
Accession NZ_CP056386
Organism Klebsiella pneumoniae strain RHBSTW-00610

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag HV247_RS25970 Protein ID WP_004197633.1
Coordinates 12634..12939 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV247_RS25975 Protein ID WP_006788213.1
Coordinates 12941..13159 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV247_RS25935 8213..9193 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
HV247_RS25940 9296..9409 + 114 WP_014343462.1 hypothetical protein -
HV247_RS25945 9538..9795 + 258 WP_009310077.1 hypothetical protein -
HV247_RS25950 9853..10632 - 780 WP_023287113.1 site-specific integrase -
HV247_RS25955 10830..11846 - 1017 WP_009309921.1 hypothetical protein -
HV247_RS25960 11880..12215 - 336 WP_009309920.1 hypothetical protein -
HV247_RS25965 12265..12405 - 141 WP_162898808.1 hypothetical protein -
HV247_RS25970 12634..12939 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
HV247_RS25975 12941..13159 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV247_RS25980 13756..13986 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV247_RS25985 13983..14399 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
HV247_RS25990 14440..15318 - 879 WP_032427228.1 restriction endonuclease -
HV247_RS25995 15980..17257 + 1278 WP_009309915.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..252882 252882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T163364 WP_004197633.1 NZ_CP056386:c12939-12634 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T163364 NZ_CP072551:c24893-24537 [Acinetobacter lwoffii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTGGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT163364 WP_006788213.1 NZ_CP056386:c13159-12941 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT163364 NZ_CP072551:c24544-24242 [Acinetobacter lwoffii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTTGAAATTTCTCAAAAAGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References