Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5221586..5222211 Replicon chromosome
Accession NZ_CP056385
Organism Klebsiella pneumoniae strain RHBSTW-00610

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV247_RS25510 Protein ID WP_002882817.1
Coordinates 5221586..5221969 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV247_RS25515 Protein ID WP_004150355.1
Coordinates 5221969..5222211 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV247_RS25495 5218952..5219854 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV247_RS25500 5219851..5220486 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV247_RS25505 5220483..5221412 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV247_RS25510 5221586..5221969 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV247_RS25515 5221969..5222211 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV247_RS25520 5222416..5223333 + 918 WP_012737090.1 alpha/beta hydrolase -
HV247_RS25525 5223348..5224289 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV247_RS25530 5224334..5224771 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV247_RS25535 5224768..5225628 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV247_RS25540 5225622..5226221 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T163363 WP_002882817.1 NZ_CP056385:c5221969-5221586 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163363 NZ_CP072550:c243204-242917 [Acinetobacter lwoffii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163363 WP_004150355.1 NZ_CP056385:c5222211-5221969 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163363 NZ_CP072550:c242930-242616 [Acinetobacter lwoffii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References