Detailed information of TA system TA163361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4596390..4597200
• Replicon: chromosome
• Accession: NZ_CP056385
• Organism: Klebsiella pneumoniae strain RHBSTW-00610

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV247_RS22610
• Protein ID: WP_032412932.1
• Coordinates: 4596390..4596923 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HV247_RS22615
• Protein ID: WP_002887278.1
• Coordinates: 4596934..4597200 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV247_RS22605	4595221..4596342	+	1122	WP_012737335.1	cupin domain-containing protein	-
HV247_RS22610	4596390..4596923	-	534	WP_032412932.1	GNAT family N-acetyltransferase	Toxin
HV247_RS22615	4596934..4597200	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HV247_RS22620	4597303..4598736	-	1434	WP_032412930.1	Cu(+)/Ag(+) sensor histidine kinase	-
HV247_RS22625	4598726..4599409	-	684	WP_032425532.1	copper response regulator transcription factor CusR	-
HV247_RS22630	4599581..4600966	+	1386	WP_032414381.1	efflux transporter outer membrane subunit	-
HV247_RS22635	4600984..4601328	+	345	WP_023287080.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19858.69 Da
• Isoelectric Point: 5.2614

>T163361 WP_032412932.1 NZ_CP056385:c4596923-4596390 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHFKRQHDGQILRGYALLSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T163361 NZ_CP072550:c240058-239777 [Acinetobacter lwoffii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT163361 WP_002887278.1 NZ_CP056385:c4597200-4596934 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT163361 NZ_CP072550:c240361-240059 [Acinetobacter lwoffii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1